Lcp1 lymphocyte cytosolic protein 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Lcp1provided by MGI
Official Full Name: lymphocyte cytosolic protein 1provided by MGI
Primary source: MGI:MGI:104808
See related: Ensembl:ENSMUSG00000021998 AllianceGenome:MGI:104808
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Pls2; pp65; LCP-1; D14Ertd310e
Summary: Enables actin filament binding activity and identical protein binding activity. Acts upstream of or within actin filament bundle assembly. Located in several cellular components, including actin filament; phagocytic cup; and ruffle. Is expressed in several structures, including brain; cardiovascular system; egg cylinder; female reproductive system; and gut. Orthologous to human LCP1 (lymphocyte cytosolic protein 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in placenta adult (RPKM 28.5), spleen adult (RPKM 23.6) and 17 other tissues See more
Orthologs: human all
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Genomic context

Location:: 14 D3; 14 39.63 cM

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (75368570..75468282)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (75131105..75230842)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

L-plastin enhances NLRP3 inflammasome assembly and bleomycin-induced lung fibrosis.
Title: L-plastin enhances NLRP3 inflammasome assembly and bleomycin-induced lung fibrosis.
Neutrophil L-Plastin Controls Ocular Paucibacteriality and Susceptibility to Keratitis.
Title: Neutrophil L-Plastin Controls Ocular Paucibacteriality and Susceptibility to Keratitis.
A novel regulatory role of TRAPPC9 in L-plastin-mediated osteoclast actin ring formation.
Title: A novel regulatory role of TRAPPC9 in L-plastin-mediated osteoclast actin ring formation.
Among the proteins found to preferentially bind clasped rather than the isolated alphaM and beta2 subunits was L-plastin (LCP1, also known as plastin-2), which binds to and maintains the inactive state of alphaMbeta2 integrin in vivo and thereby regulates leukocyte adhesion to integrin ligands under flow.
Title: LCP1 preferentially binds clasped αMβ2 integrin and attenuates leukocyte adhesion under flow.
Our data suggest that targeted disruption of L-plastin increases trabecular bone volume, and phosphorylation of Ser5 in L-plastin in the Lrrk1 signaling pathway may in part contribute to actin assembly in mature osteoclasts.
Title: LRRK1 regulation of actin assembly in osteoclasts involves serine 5 phosphorylation of L-plastin.
It is shown that TNF-alpha signaling regulates the phosphorylation of serine-5 and -7 in L-plastin which increases the actin bundling capacity of L-plastin and hence the formation of nascent sealing zones in mouse osteoclasts.
Title: L-plastin phosphorylation regulates the early phase of sealing ring formation by actin bundling process in mouse osteoclasts.
the actin-bundling protein L-plastin (LPL) is required for the perinatal development of alveolar macrophages.
Title: Alveolar macrophage development in mice requires L-plastin for cellular localization in alveoli.
Results identify L-plastin as a key effector of Mst1 and establish a novel mechanism linking a signaling intermediate to an actin-binding protein critical to T cell migration.
Title: Mst1 Kinase Regulates the Actin-Bundling Protein L-Plastin To Promote T Cell Migration.
L-plastin modulates both T- and B-cell function during the germinal center reaction and the production of T-cell-dependent antibody responses.
Title: Intrinsic T- and B-cell defects impair T-cell-dependent antibody responses in mice lacking the actin-bundling protein L-plastin.
L-plastin may participate in signaling that enables lymphocyte transmigration
Title: The actin-bundling protein L-plastin is essential for marginal zone B cell development.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (5) 1 citation
Endonuclease-mediated (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Lcp1 (e-PCR), detects polymorphism
- UniSTS:499096

16.MMHAP57FLF1.seq (e-PCR)
- UniSTS:123310

RH126802 (e-PCR)
- UniSTS:167131

AW536232 (e-PCR)
- UniSTS:180701

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables GTPase binding	ISO Inferred from Sequence Orthology more info
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin filament binding	IDA Inferred from Direct Assay more info	PubMed
enables actin filament binding	ISO Inferred from Sequence Orthology more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables integrin binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in T cell activation involved in immune response	ISO Inferred from Sequence Orthology more info
involved_in actin filament bundle assembly	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within actin filament bundle assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in actin filament bundle assembly	ISO Inferred from Sequence Orthology more info
involved_in actin filament network formation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell migration	ISO Inferred from Sequence Orthology more info
involved_in extracellular matrix disassembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of podosome assembly	ISO Inferred from Sequence Orthology more info
involved_in protein kinase A signaling	ISO Inferred from Sequence Orthology more info
involved_in regulation of intracellular protein transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to wounding	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in actin cytoskeleton	ISO Inferred from Sequence Orthology more info
is_active_in actin filament	IBA Inferred from Biological aspect of Ancestor more info
located_in actin filament	IDA Inferred from Direct Assay more info	PubMed
located_in actin filament	ISO Inferred from Sequence Orthology more info
is_active_in actin filament bundle	IBA Inferred from Biological aspect of Ancestor more info
located_in actin filament bundle	ISO Inferred from Sequence Orthology more info
located_in anchoring junction	IEA Inferred from Electronic Annotation more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in filopodium	ISO Inferred from Sequence Orthology more info
located_in focal adhesion	ISO Inferred from Sequence Orthology more info
located_in glial cell projection	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in phagocytic cup	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in podosome	ISO Inferred from Sequence Orthology more info
located_in ruffle	IDA Inferred from Direct Assay more info	PubMed
located_in ruffle	ISO Inferred from Sequence Orthology more info
colocalizes_with stress fiber	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: plastin-2

Names: 65 kDa macrophage protein; L-fimbrin; L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (65 kDa macrophage protein) (PP65); plastin 2, L

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001247984.2 → NP_001234913.1 plastin-2

See identical proteins and their annotated locations for NP_001234913.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.

Source sequence(s)

AC129591, AC161877

Consensus CDS

CCDS27276.1

UniProtKB/Swiss-Prot

Q3UE24, Q61233, Q8R1X3, Q9CV77

UniProtKB/TrEMBL

Q3TJX0, Q3U6Z1, Q3U9M7

Related

ENSMUSP00000121201.2, ENSMUST00000124499.8

Conserved Domains (5) summary

cd00051
Location:13 → 79

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

cd21292
Location:98 → 242

CH_PLS_rpt1; first calponin homology (CH) domain found in the plastin family

cd21327
Location:251 → 375

CH_PLS2_rpt2; second calponin homology (CH) domain found in plastin-2

cd21333
Location:511 → 625

CH_PLS2_rpt4; fourth calponin homology (CH) domain found in plastin-2

cl00030
Location:374 → 507

CH_SF; calponin homology (CH) domain superfamily
NM_001424512.1 → NP_001411441.1 plastin-2

Status: VALIDATED

Source sequence(s)

AC129591, AC161877

UniProtKB/Swiss-Prot

Q3UE24, Q61233, Q8R1X3, Q9CV77
NM_001424513.1 → NP_001411442.1 plastin-2

Status: VALIDATED

Source sequence(s)

AC129591, AC161877

UniProtKB/Swiss-Prot

Q3UE24, Q61233, Q8R1X3, Q9CV77
NM_001424514.1 → NP_001411443.1 plastin-2

Status: VALIDATED

Source sequence(s)

AC129591, AC161877

UniProtKB/Swiss-Prot

Q3UE24, Q61233, Q8R1X3, Q9CV77
NM_001424515.1 → NP_001411444.1 plastin-2

Status: VALIDATED

Source sequence(s)

AC129591, AC161877

UniProtKB/Swiss-Prot

Q3UE24, Q61233, Q8R1X3, Q9CV77
NM_008879.5 → NP_032905.2 plastin-2

See identical proteins and their annotated locations for NP_032905.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.

Source sequence(s)

AC129591, AC161877

Consensus CDS

CCDS27276.1

UniProtKB/Swiss-Prot

Q3UE24, Q61233, Q8R1X3, Q9CV77

UniProtKB/TrEMBL

Q3TJX0, Q3U6Z1, Q3U9M7

Related

ENSMUSP00000116271.2, ENSMUST00000145303.8

Conserved Domains (5) summary

cd00051
Location:13 → 79

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

cd21292
Location:98 → 242

CH_PLS_rpt1; first calponin homology (CH) domain found in the plastin family

cd21327
Location:251 → 375

CH_PLS2_rpt2; second calponin homology (CH) domain found in plastin-2

cd21333
Location:511 → 625

CH_PLS2_rpt4; fourth calponin homology (CH) domain found in plastin-2

cl00030
Location:374 → 507

CH_SF; calponin homology (CH) domain superfamily

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000080.7 Reference GRCm39 C57BL/6J

Range

75368570..75468282

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036158507.1 → XP_036014400.1 plastin-2 isoform X1

UniProtKB/Swiss-Prot

Q3UE24, Q61233, Q8R1X3, Q9CV77

UniProtKB/TrEMBL

Q3TJX0, Q3U6Z1, Q3U9M7

Conserved Domains (5) summary

cd00051
Location:13 → 79

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

cd21292
Location:98 → 242

CH_PLS_rpt1; first calponin homology (CH) domain found in the plastin family

cd21327
Location:251 → 375

CH_PLS2_rpt2; second calponin homology (CH) domain found in plastin-2

cd21333
Location:511 → 625

CH_PLS2_rpt4; fourth calponin homology (CH) domain found in plastin-2

cl00030
Location:374 → 507

CH_SF; calponin homology (CH) domain superfamily