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    truB tRNA pseudouridine(55) synthase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947687, updated on 23-Nov-2023

    Summary

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    Gene symbol
    truB
    Gene description
    tRNA pseudouridine(55) synthase
    Primary source
    ASAP:ABE-0010404
    Locus tag
    b3166
    See related
    ECOCYC:EG11177
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3155; yhbA
    Summary
    [More information is available at EcoGene: EG11177]. TruB catalyzes pseudouridylation at position 55 in tRNA . [More information is available at EcoCyc: EG11177].
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    Genomic context

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    Sequence:
    NC_000913.3 (3311833..3312777, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene 30S ribosomal subunit protein S15 Neighboring gene 30S ribosome binding factor Neighboring gene translation initiation factor IF-2beta' Neighboring gene transcription termination/antitermination protein NusA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. RNA modification enzyme TruB is a tRNA chaperone. Keffer-Wilkes LC, et al. Proc Natl Acad Sci U S A, 2016 Dec 13. PMID 27849601, Free PMC Article
    2. Dissecting the roles of a strictly conserved tyrosine in substrate recognition and catalysis by pseudouridine 55 synthase. Phannachet K, et al. Biochemistry, 2005 Nov 29. PMID 16300397
    3. Precursor complex structure of pseudouridine synthase TruB suggests coupling of active site perturbations to an RNA-sequestering peripheral protein domain. Hoang C, et al. Protein Sci, 2005 Aug. PMID 15987897, Free PMC Article
    4. The roles of the essential Asp-48 and highly conserved His-43 elucidated by the pH dependence of the pseudouridine synthase TruB. Hamilton CS, et al. Arch Biochem Biophys, 2005 Jan 1. PMID 15581587
    5. The pseudouridine synthases: revisiting a mechanism that seemed settled. Spedaliere CJ, et al. J Am Chem Soc, 2004 Oct 13. PMID 15469254

    See all (27) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. we prove the tRNA chaperone activity of the pseudouridine synthase TruB, reveal its molecular mechanism, and demonstrate its importance for cellular fitness
      Title: RNA modification enzyme TruB is a tRNA chaperone.
    2. The single-round rate constant of pseudouridylation by TruB was determined to be 0.5 sec(-1).
      Title: Pre-steady-state kinetic analysis of the three Escherichia coli pseudouridine synthases TruB, TruA, and RluA reveals uniformly slow catalysis.
    3. N-terminus verified by Edman degradation on mature peptide
      Title: Purification, cloning, and properties of the tRNA psi 55 synthase from Escherichia coli.
    4. Structural model of TruB in complex with a substrate RNA, suggests communication between the active site and the RNA-sequestering peripheral protein domain.
      Title: Precursor complex structure of pseudouridine synthase TruB suggests coupling of active site perturbations to an RNA-sequestering peripheral protein domain.
    Submit: New GeneRIF Correction

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables catalytic activity, acting on RNA IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables pseudouridine synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables pseudouridine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables tRNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tRNA pseudouridine synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tRNA pseudouridine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA modification IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in RNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA pseudouridine synthesis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in ncRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in pseudouridine synthesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within tRNA folding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA modification IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA pseudouridine synthesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within tRNA pseudouridine synthesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    tRNA pseudouridine(55) synthase
    NP_417635.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3311833..3312777 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417635.1 tRNA pseudouridine(55) synthase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417635.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A7L5V8K6, A0A8S7Y6K5
      Conserved Domains (1) summary
      PRK05033
      Location:3 → 314
      truB; tRNA pseudouridine synthase B; Provisional
    Nucleotide Protein
    Heading Accession and Version

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