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    dna-2 DNA replication ATP-dependent helicase/nuclease [ Caenorhabditis elegans ]

    Gene ID: 174809, updated on 23-Nov-2023

    Summary

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    Gene symbol
    dna-2
    Gene description
    DNA replication ATP-dependent helicase/nuclease
    Primary source
    WormBase:WBGene00001016
    Locus tag
    CELE_F43G6.1
    See related
    AllianceGenome:WB:WBGene00001016
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Caenorhabditis elegans (strain: Bristol N2)
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
    Summary
    Enables 5'-3' DNA helicase activity; single-stranded DNA endodeoxyribonuclease activity; and single-stranded DNA helicase activity. Involved in DNA metabolic process. Located in nucleus. Is expressed in gonad and oocyte. Human ortholog(s) of this gene implicated in Seckel syndrome 8; autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions 6; isolated growth hormone deficiency type IA; and mitochondrial myopathy. Orthologous to human DNA2 (DNA replication helicase/nuclease 2). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    Location:
    chromosome: II
    Exon count:
    10
    Sequence:
    Chromosome: II; NC_003280.10 (11778430..11782396)

    Chromosome II - NC_003280.10Genomic Context describing neighboring genes Neighboring gene ATP synthase subunit e, mitochondrial Neighboring gene Protein PAT1 homolog 1 Neighboring gene Neuropeptide-Like Protein Neighboring gene pseudo Neighboring gene ncRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003280.10

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Caenorhabditis elegans DNA-2 helicase/endonuclease plays a vital role in maintaining genome stability, morphogenesis, and life span. Lee MH, et al. Biochem Biophys Res Commun, 2011 Apr 15. PMID 21414295
    2. Caenorhabditis elegans dna-2 is involved in DNA repair and is essential for germ-line development. Lee MH, et al. FEBS Lett, 2003 Dec 4. PMID 14644423
    3. Enzymatic properties of the Caenorhabditis elegans Dna2 endonuclease/helicase and a species-specific interaction between RPA and Dna2. Kim DH, et al. Nucleic Acids Res, 2005. PMID 15745997, Free PMC Article
    4. Dna2 requirement for normal reproduction of Caenorhabditis elegans is temperature-dependent. Lee KH, et al. Mol Cells, 2003 Feb 28. PMID 12661765
    5. The Caenorhabditis elegans WRN helicase promotes double-strand DNA break repair by mediating end resection and checkpoint activation. Ryu JS, et al. FEBS Lett, 2017 Jul. PMID 28640365

    See all (14) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Cedna-2 contributes to embryonic viability, the morphogenesis of both late-stage embryos and male sensory rays, and normal life span.
      Title: Caenorhabditis elegans DNA-2 helicase/endonuclease plays a vital role in maintaining genome stability, morphogenesis, and life span.
    2. A comparative study of the enzymatic properties of C.elegans Dna2 and S.cerevisiae Dna2 and their interaction with replication-protein A.
      Title: Enzymatic properties of the Caenorhabditis elegans Dna2 endonuclease/helicase and a species-specific interaction between RPA and Dna2.
    3. Dna2 localizes to the nuclei of C. elegans oocytes & early embryos. Homozygous deletion causes temperature-dependent reduced brood size & embryonic lethality, delayed cell-division.
      Title: Dna2 requirement for normal reproduction of Caenorhabditis elegans is temperature-dependent.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by WormBase

    Function Evidence Code Pubs
    enables 4 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables 5'-3' DNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 5'-flap endonuclease activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 5'-flap endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables catalytic activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables iron-sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables single-stranded DNA endodeoxyribonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables single-stranded DNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables single-stranded DNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA damage response IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA duplex unwinding IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication, Okazaki fragment processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in replication fork reversal IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in chromosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    DNA replication ATP-dependent helicase/nuclease
    NP_496516.1
    • Confirmed by transcript evidence

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003280.10 Reference assembly

      Range
      11778430..11782396
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_064115.4 → NP_496516.1  DNA replication ATP-dependent helicase/nuclease [Caenorhabditis elegans]

      See identical proteins and their annotated locations for NP_496516.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      Q9U3F2
      Conserved Domains (5) summary
      TIGR00376
      Location:480 → 1062
      TIGR00376; DNA helicase, putative
      pfam08696
      Location:122 → 325
      Dna2; DNA replication factor Dna2
      pfam13087
      Location:834 → 1044
      AAA_12; AAA domain
      cl00641
      Location:294 → 430
      Cas4_I-A_I-B_I-C_I-D_II-B; CRISPR/Cas system-associated protein Cas4
      cl21455
      Location:650 → 861
      P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

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