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    Mthfs 5, 10-methenyltetrahydrofolate synthetase [ Mus musculus (house mouse) ]

    Gene ID: 107885, updated on 5-Mar-2024

    Summary

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    Official Symbol
    Mthfsprovided by MGI
    Official Full Name
    5, 10-methenyltetrahydrofolate synthetaseprovided by MGI
    Primary source
    MGI:MGI:1340032
    See related
    Ensembl:ENSMUSG00000066442 AllianceGenome:MGI:1340032
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    1110034I12Rik; 2310020H23Rik
    Summary
    Predicted to enable 5-formyltetrahydrofolate cyclo-ligase activity; anion binding activity; and double-stranded DNA binding activity. Predicted to be involved in DNA geometric change; folic acid-containing compound metabolic process; and termination of mitochondrial transcription. Located in mitochondrion. Is expressed in several structures, including alimentary system; genitourinary system; integumental system; nervous system; and sensory organ. Orthologous to several human genes including ST20-MTHFS (ST20-MTHFS readthrough). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Ubiquitous expression in liver E18 (RPKM 15.5), liver adult (RPKM 15.0) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    9 E3.1; 9 47.24 cM
    Exon count:
    4
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 9 NC_000075.7 (89093243..89122278)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 9 NC_000075.6 (89211190..89240225)

    Chromosome 9 - NC_000075.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer mm9_chr9:89090619-89090919 Neighboring gene STARR-positive B cell enhancer ABC_E6785 Neighboring gene predicted gene, 40544 Neighboring gene STARR-positive B cell enhancer ABC_E8305 Neighboring gene STARR-positive B cell enhancer ABC_E11418 Neighboring gene B cell leukemia/lymphoma 2 related protein A1b Neighboring gene STARR-seq mESC enhancer starr_24872 Neighboring gene STARR-seq mESC enhancer starr_24873 Neighboring gene predicted gene, 40543 Neighboring gene STARR-seq mESC enhancer starr_24874 Neighboring gene STARR-seq mESC enhancer starr_24875 Neighboring gene STARR-seq mESC enhancer starr_24876 Neighboring gene predicted gene, 57551

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. 5,10-Methenyltetrahydrofolate synthetase activity is increased in tumors and modifies the efficacy of antipurine LY309887. Field MS, et al. Arch Biochem Biophys, 2009 Jan 15. PMID 19022216, Free PMC Article
    2. Inhibition of 5,10-methenyltetrahydrofolate synthetase. Field MS, et al. Arch Biochem Biophys, 2007 Feb 15. PMID 17250800, Free PMC Article
    3. Methenyltetrahydrofolate synthetase is a high-affinity catecholamine-binding protein. Anguera MC, et al. Arch Biochem Biophys, 2006 Nov 15. PMID 17055997, Free PMC Article
    4. Regulation of de novo purine biosynthesis by methenyltetrahydrofolate synthetase in neuroblastoma. Field MS, et al. J Biol Chem, 2006 Feb 17. PMID 16365037
    5. Mthfs is an Essential Gene in Mice and a Component of the Purinosome. Field MS, et al. Front Genet, 2011. PMID 22303332, Free PMC Article

    See all (24) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Expression of the MTHFS cDNA in MCF-7 cells increased the concentration of NADA required to deplete cellular folate.
      Title: Methenyltetrahydrofolate synthetase is a high-affinity catecholamine-binding protein.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Gene trapped (1) 
    • Endonuclease-mediated (1) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables 5-formyltetrahydrofolate cyclo-ligase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 5-formyltetrahydrofolate cyclo-ligase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables ATP binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables double-stranded DNA binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables folic acid binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA geometric change ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in DNA-templated transcription termination ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in folic acid catabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in folic acid-containing compound biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in glutamate metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in termination of mitochondrial transcription ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in tetrahydrofolate interconversion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in tetrahydrofolate interconversion ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in tetrahydrofolate metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in mitochondrial matrix ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in mitochondrial nucleoid ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in mitochondrion HDA PubMed 
    is_active_in mitochondrion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in mitochondrion ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    5-formyltetrahydrofolate cyclo-ligase
    Names
    5,10-methenyl-tetrahydrofolate synthetase
    methenyl-THF synthetase
    NP_001346922.1
    NP_081105.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001359993.1 → NP_001346922.1  5-formyltetrahydrofolate cyclo-ligase isoform 2

      Status: VALIDATED

      Source sequence(s)
      AC135634, CT030686
      Conserved Domains (1) summary
      pfam01812
      Location:1 → 141
      5-FTHF_cyc-lig; 5-formyltetrahydrofolate cyclo-ligase family

    2. NM_026829.2 → NP_081105.1  5-formyltetrahydrofolate cyclo-ligase isoform 1

      See identical proteins and their annotated locations for NP_081105.1

      Status: VALIDATED

      Source sequence(s)
      AK004092
      Consensus CDS
      CCDS23394.1
      UniProtKB/Swiss-Prot
      B2KF82, Q9D110
      UniProtKB/TrEMBL
      Q3TYX5
      Related
      ENSMUSP00000082354.7, ENSMUST00000085256.8
      Conserved Domains (1) summary
      pfam01812
      Location:10 → 198
      5-FTHF_cyc-lig; 5-formyltetrahydrofolate cyclo-ligase family

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000075.7 Reference GRCm39 C57BL/6J

      Range
      89093243..89122278
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9D110.2 GenPept UniProtKB/Swiss-Prot:Q9D110

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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