Location on the chromosome of Escherichia coli of genes governing purine metabolism. Adenosine deaminase (add), guanosine kinase (gsk) and hypoxanthine phosphoribosyltransferase (hpt)

Mol Gen Genet. 1975 Dec 30;143(1):85-91. doi: 10.1007/BF00269424.

Abstract

Genes coding for enzymes functioning in purine salvage pathways have been located on the chromosome of Escherichia coli. The gene add encoding adenosine deaminase was located by transduction at 31 min, the gene order was established to be man-uidA-add-aroD. A deletion covering man-uidA-add was obtained. The gene gsk encoding guanosine kinase was cotransducible with purE and shown to be located at 13 min. The gene hpt encoding hypoxanthine phosphoribosyltransferase was cotransducible with tonA indicating a location at 3 min. The location of the gene gpt encoding guanine (xanthine) phosphoribosyltransferase in the proA-proB region was confirmed.

MeSH terms

  • Adenine Phosphoribosyltransferase
  • Adenosine / metabolism
  • Adenosine Deaminase / biosynthesis*
  • Chromosome Deletion
  • Chromosome Mapping
  • Chromosomes, Bacterial*
  • Conjugation, Genetic
  • Escherichia coli / enzymology*
  • Escherichia coli / metabolism
  • Genes*
  • Guanine
  • Guanosine / metabolism
  • Hypoxanthine Phosphoribosyltransferase / biosynthesis*
  • Hypoxanthines / metabolism
  • Inosine / metabolism
  • Mutation
  • Nucleoside Deaminases / biosynthesis*
  • Pentosyltransferases / biosynthesis*
  • Purines / metabolism
  • Recombination, Genetic
  • Transduction, Genetic
  • Transferases
  • Xanthines

Substances

  • Hypoxanthines
  • Purines
  • Xanthines
  • Guanosine
  • Inosine
  • Guanine
  • Transferases
  • Pentosyltransferases
  • Adenine Phosphoribosyltransferase
  • Hypoxanthine Phosphoribosyltransferase
  • Nucleoside Deaminases
  • Adenosine Deaminase
  • Adenosine