Abstract
We have developed a reverse-transcriptase polymerase chain reaction assay for rapid detection of Langat (LGT) virus, a flavivirus that is closely related to the highly pathogenic tick-borne encephalitis (TBE) viruses. Unlike TBE viruses, LGT virus exhibits a significantly lower virulence for man. The assay serves as a safe alternative for the development and optimization of specific assays for the highly pathogenic subtypes of TBE viruses that are endemic throughout much of Europe, the former Soviet Union, and China.
MeSH terms
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Animals
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Base Sequence
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DNA Primers
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DNA, Complementary / analysis
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DNA, Complementary / genetics
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Encephalitis Viruses, Tick-Borne / chemistry
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Encephalitis Viruses, Tick-Borne / genetics
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Encephalitis Viruses, Tick-Borne / isolation & purification*
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Encephalitis Viruses, Tick-Borne / pathogenicity
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Humans
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Molecular Sequence Data
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Polymerase Chain Reaction*
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RNA, Viral / genetics*
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RNA-Directed DNA Polymerase
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Sensitivity and Specificity
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Sequence Alignment
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Sequence Analysis, DNA
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Vero Cells
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Virulence
Substances
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DNA Primers
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DNA, Complementary
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RNA, Viral
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RNA-Directed DNA Polymerase