Analysis of high-molecular-weight ribonucleic acid associated with intracisternal A particles

J Virol. 1973 Feb;11(2):287-98. doi: 10.1128/JVI.11.2.287-298.1973.

Abstract

Intracisternal A particles, known primarily for their association with various tumors, have been shown to contain high-molecular-weight (HMW) ribonucleic acid (RNA) by velocity centrifugation, using linear glycerol gradients. This HMW RNA is sensitive to ribonuclease digestion and alkali treatment but is resistant to Pronase treatment. By a double-labeling experiment, HMW RNA was shown to be intrinsic to intracisternal A particles and not to have resulted from cytoplasmic polysomal RNA aggregation. By a reconstitution experiment, it was determined that the results were not due to C-type virus contamination. The synthesis of HMW RNA in intracisternal A particles is inhibited by actinomycin D and ethidium bromide. These observations emphasize that there are probably some taxonomic relationships between intracisternal A particles and oncogenic RNA viruses.

MeSH terms

  • Animals
  • Bone Marrow Cells
  • Carbon Isotopes
  • Cell Line
  • Cells, Cultured
  • Centrifugation, Density Gradient
  • Complement Fixation Tests
  • Dactinomycin / pharmacology
  • Ethidium / pharmacology
  • Female
  • Fibroblasts
  • Inclusion Bodies, Viral*
  • Mice
  • Mice, Inbred BALB C
  • Microscopy, Electron
  • Molecular Weight
  • Neoplasm Transplantation
  • Neuroblastoma
  • Oncogenic Viruses / classification
  • Plasmacytoma
  • RNA Viruses / classification
  • RNA, Viral / analysis*
  • RNA, Viral / biosynthesis
  • Tritium
  • Viral Plaque Assay

Substances

  • Carbon Isotopes
  • RNA, Viral
  • Tritium
  • Dactinomycin
  • Ethidium