Abstract
1-Aminooxy-3-aminopropane (APA) was shown to be a potent competitive inhibitor (Ki = 1.0 nM) of partially purified Escherichia coli ornithine decarboxylase. APA did not inhibit S-adenosyl-L-methionine decarboxylase and spermidine from E. coli. S-(5'-Deoxy-5'-adenosyl)methylthioethylhydroxylamine (AMA), which is a structural analogue of decarboxylated S-adenosyl-L-methionine, was for the first time shown to be an irreversible inhibitor of bacterial S-adenosyl-L-methionine decarboxylase and a competitive inhibitor (Ki = 47 microM) of bacterial ornithine decarboxylase. AMA had no effect on spermidine synthase.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosylmethionine Decarboxylase / antagonists & inhibitors*
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Carboxy-Lyases / antagonists & inhibitors*
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Deoxyadenosines / analogs & derivatives*
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Deoxyadenosines / pharmacology
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Dose-Response Relationship, Drug
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Escherichia coli / enzymology*
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Ornithine Decarboxylase Inhibitors*
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Polyamines / biosynthesis*
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Propylamines / pharmacology*
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Spermidine Synthase / metabolism
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Time Factors
Substances
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Deoxyadenosines
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Ornithine Decarboxylase Inhibitors
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Polyamines
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Propylamines
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S-(5'-deoxyadenosyl-5')-S-methylthioethylhydroxylamine
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1-aminooxy-3-aminopropane
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Spermidine Synthase
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Carboxy-Lyases
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Adenosylmethionine Decarboxylase