The effects of 1-aminooxy-3-aminopropane and S-(5'-deoxy-5'-adenosyl)methylthioethylhydroxylamine on ornithine decarboxylase and S-adenosyl-L-methionine decarboxylase from Escherichia coli

FEBS Lett. 1986 Jul 7;202(2):323-6. doi: 10.1016/0014-5793(86)80710-4.

Abstract

1-Aminooxy-3-aminopropane (APA) was shown to be a potent competitive inhibitor (Ki = 1.0 nM) of partially purified Escherichia coli ornithine decarboxylase. APA did not inhibit S-adenosyl-L-methionine decarboxylase and spermidine from E. coli. S-(5'-Deoxy-5'-adenosyl)methylthioethylhydroxylamine (AMA), which is a structural analogue of decarboxylated S-adenosyl-L-methionine, was for the first time shown to be an irreversible inhibitor of bacterial S-adenosyl-L-methionine decarboxylase and a competitive inhibitor (Ki = 47 microM) of bacterial ornithine decarboxylase. AMA had no effect on spermidine synthase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosylmethionine Decarboxylase / antagonists & inhibitors*
  • Carboxy-Lyases / antagonists & inhibitors*
  • Deoxyadenosines / analogs & derivatives*
  • Deoxyadenosines / pharmacology
  • Dose-Response Relationship, Drug
  • Escherichia coli / enzymology*
  • Ornithine Decarboxylase Inhibitors*
  • Polyamines / biosynthesis*
  • Propylamines / pharmacology*
  • Spermidine Synthase / metabolism
  • Time Factors

Substances

  • Deoxyadenosines
  • Ornithine Decarboxylase Inhibitors
  • Polyamines
  • Propylamines
  • S-(5'-deoxyadenosyl-5')-S-methylthioethylhydroxylamine
  • 1-aminooxy-3-aminopropane
  • Spermidine Synthase
  • Carboxy-Lyases
  • Adenosylmethionine Decarboxylase