Psychoactive drugs citalopram and mirtazapine caused oxidative stress and damage of feeding behavior in Daphnia magna

As the emerging contaminants, the environmental risks of drug-derived pollutants have attracted extensive attention. Citalopram (CTP) and mirtazapine (MTP) are commonly used as modern antidepressant drugs. Previous studies had proved that CTP and MTP entered the aquatic environment, but less reported the negative effects of the drugs on aquatic organisms. Herein, the effects on the feeding rate of Daphnia magna (D. magna) induced by psychotropic drugs CTP and MTP were investigated, which the possible mechanisms were analyzed with the oxidative stress and damage. Generally, the feeding rates of exposed D. magna under all concentrations of CTP and 1.03 mg/L of MTP were significantly decreased after exposure (p < 0.05 or p < 0.01). The inhibitory effect of CTP on the feeding rate of D. magna was time- and dose-dependent. The levels of reactive oxygen species (ROS) were particularly increased in D. magna after CTP and MTP exposure (p < 0.05 or p < 0.01). The level of antioxidant molecules glutathione S-transferase (GST) and the activity of scavenging enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) of D. magna were increased (p < 0.05 or p < 0.01). In consequence, the levels of malondialdehyde (MDA), protein carbonyl, and 8-hydroxydeoxyguanosine (8-OHdG) were increased (p < 0.05 or p < 0.01), which indicated oxidative damage caused by MTP and CTP, due to the imbalance of antioxidative stress system. These findings indicated that psychoactive drugs posed a high toxic threat to the aquatic organisms, and the aquatic environmental risks caused by using psychoactive drugs deserve more attention.