Effect of Pholiota nameko Polysaccharides Inhibiting Methylglyoxal-Induced Glycation Damage In Vitro

His Lin; Ting-Yun Lin; Jer-An Lin; Kuan-Chen Cheng; Shella Permatasari Santoso; Chun-Hsu Chou; Chang-Wei Hsieh

doi:10.3390/antiox10101589

Effect of Pholiota nameko Polysaccharides Inhibiting Methylglyoxal-Induced Glycation Damage In Vitro

Antioxidants (Basel). 2021 Oct 10;10(10):1589. doi: 10.3390/antiox10101589.

Authors

His Lin¹, Ting-Yun Lin¹, Jer-An Lin², Kuan-Chen Cheng^{3

4

5

6}, Shella Permatasari Santoso^{7

8}, Chun-Hsu Chou⁹, Chang-Wei Hsieh^{1

6}

Affiliations

¹ Department of Food Science and Biotechnology, National Chung Hsing University, 145 Xingda Rd., South Dist., Taichung City 40227, Taiwan.
² Graduate Institute of Food Safety, National Chung Hsing University, 145 Xingda Rd., South Dist., Taichung City 40227, Taiwan.
³ Institute of Biotechnology, National Taiwan University, Taipei 10617, Taiwan.
⁴ Graduate Institute of Food Science Technology, National Taiwan University, Taipei 10617, Taiwan.
⁵ Department of Optometry, Asia University, 500, Lioufeng Rd., Wufeng, Taichung City 41354, Taiwan.
⁶ Department of Medical Research, China Medical University Hospital, Taichung City 406040, Taiwan.
⁷ Department of Chemical Engineering, Widya Mandala Surabaya Catholic University, Kalijudan 37, Surabaya 60114, Indonesia.
⁸ Department of Chemical Engineering, National Taiwan University of Science and Technology, Keelung Rd. 43, Da'an Dist., Taipei 10607, Taiwan.
⁹ Dr Jou Biotech Co., Ltd., No. 21, Lugong S. 2nd Rd., Lukang Township, Changhua 505, Taiwan.

Abstract

Advanced glycation end products (AGEs) can induce oxidative stress and inflammation. AGEs are major risk factors for the development of many aging-related diseases, such as cancer and diabetes. In this study, Pholiota nameko polysaccharides (PNPs) were prepared from water extract of P. nameko via graded alcohol precipitation (40%, 60%, and 80% v/v). We explored the in vitro antiglycation ability of the PNPs and inhibition of methylglyoxal (MG)-induced Hs68 cell damage. In a bovine serum albumin (BSA) glycation system, PNPs significantly inhibited the formation of Amadori products. Fluorescence spectrophotometry revealed that the PNPs trapped MG and reduced MG-induced changes in functional groups (carbonyl and ε-NH₂) in the BSA. Pretreating Hs68 cells with PNPs enhanced the cell survival rate and protected against MG-induced cell damage. This was due to decreased intracellular ROS content. PNPs thus mitigate skin cell damage and oxidative stress resulting from glycation stress, making them a potential raw material for antiaging-related skincare products.

Keywords: Pholiota nameko; advanced glycation end products; antiglycation; cell aging; glycation stress; human dermal fibroblasts; polysaccharide.

Grants and funding

108-2622-B-005-003 -CC2/Ministry of Science and Technology, Taiwan