Quantitatively determining the somatic cell count of raw milk using dielectric spectra and support vector regression

J Dairy Sci. 2022 Jan;105(1):772-781. doi: 10.3168/jds.2021-20828. Epub 2021 Sep 30.

Abstract

To investigate the potential of dielectric spectroscopy in quantitatively determining the somatic cell count (SCC) of raw milk, the dielectric spectra of 301 raw milk samples at different SCC were collected using coaxial probe technology in the frequency range of 20 to 4,500 MHz. Standard normal variate, Mahalanobis distance, and joint x-y distances sample division were used to pretreat spectra, detect outliers, and divide samples, respectively. Principal component analysis and variable importance in projection (VIP) methods were used to reduce data dimension and select characteristic variables (CVR), respectively. The full spectra, 16 principal components obtained by principal component analysis, and 86 CVR selected by VIP were used as inputs, respectively, to establish different support vector regression models. The results showed that the nonlinear support vector regression models based on the full spectra and selected CVR using VIP had the best prediction performance, with the standard error of prediction and residual predictive deviation of 0.19 log SCC/mL and 2.37, respectively. The study provided a novel method for online or in situ detection of the SCC of raw milk in production, processing, and consumption.

Keywords: dielectric property; milk; quantitative analysis; somatic cell count.

MeSH terms

  • Animals
  • Cell Count / veterinary
  • Dielectric Spectroscopy* / veterinary
  • Milk*