A TICT + AIE based fluorescent probe for ultrafast response of hypochlorite in living cells and mouse

Spectrochim Acta A Mol Biomol Spectrosc. 2021 Jul 15:256:119735. doi: 10.1016/j.saa.2021.119735. Epub 2021 Mar 23.

Abstract

Hypochlorite (HClO/ClO-), an important reactive oxygen species (ROS), plays a significant role in the human immune system. Thus, developing a fast and efficient method for detecting ClO- is quite necessary. Herein, we designed and synthesized a fluorescent probe TPB-CN based on twisted intramolecular charge transfer (TICT) and aggregation-induced emission (AIE) characteristics. The probe could respond to ClO- with an ultrafast response velocity (<2 s). The detection limit was calculated to be 6.198 nM. In addition, probe TPB-CN was successfully applied for detecting ClO- in living cells and mouse.

Keywords: Aggregation-induced emission (AIE); Fluorescent probe; Hypochlorite; Twisted intramolecular charge transfer (TICT); Ultrafast response.

MeSH terms

  • Animals
  • Fluorescent Dyes*
  • HeLa Cells
  • Humans
  • Hypochlorous Acid*
  • Mice
  • RAW 264.7 Cells

Substances

  • Fluorescent Dyes
  • Hypochlorous Acid