Electrochemical detection of methyl parathion via a novel biosensor tailored on highly biocompatible electrochemically reduced graphene oxide-chitosan-hemoglobin coatings

A quick electrochemical sensing tool by utilizing novel bioelectrode based on redox active protein hemoglobin (Hb) has been offered here for the determination of methylparathion (MP). The bioelectrode has been designed by immobilizing Hb on electrochemically reduced graphene oxide-chitosan (ERGO-CS/Hb/FTO) based biocompatible coatings. Fourier transform-infrared analyses (FTIR), field emission scanning electron microscopy (FESEM), UV-visible and electrochemical characterization reveal the successful grafting of ERGO-CS/Hb/FTO. A detailed impedimetric analysis shows low charge transfer resistance (R_CT) and solution resistance (Rs) for the fabricated biosensor, thus pointing towards improved electrochemical performance and sensitivity. In-depth elucidation of redox analysis has been presented in terms of surface concentration of redox moiety (2.92 × 10^-9 mol cm^-2) and heterogeneous electron transfer rate constant (0.0032 s^-1) which indicate enhanced surface coverage and better charge transfer properties of the proposed electrochemical biosensor. The sensor is equipped with a low limit of detection of 79.77 nM and a high sensitivity of 45.77 Acm^-2 μM^-1 with excellent reproducibility. The modified biosensor also offered its credibility towards detection of MP in vegetable samples with recovery (%) ranging from 94% to 101%. The designed biosensor hereby, evolves as a promising approach for the recognition of MP.