Virus-Induced Gene Silencing (VIGS) in Chili Pepper (Capsicum spp.)

Magda Lisette Arce-Rodríguez; Neftalí Ochoa-Alejo

doi:10.1007/978-1-0716-0751-0_3

Virus-Induced Gene Silencing (VIGS) in Chili Pepper (Capsicum spp.)

Methods Mol Biol. 2020:2172:27-38. doi: 10.1007/978-1-0716-0751-0_3.

Authors

Magda Lisette Arce-Rodríguez¹, Neftalí Ochoa-Alejo²

Affiliations

¹ Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Unidad Irapuato, Gto., Mexico.
² Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Unidad Irapuato, Gto., Mexico. neftali.ochoa@cinvestav.mx.

PMID: 32557359
DOI: 10.1007/978-1-0716-0751-0_3

Abstract

Virus-induced gene silencing (VIGS) is a transcript suppression technique that enables the functional characterization of genes in recalcitrant transformation plants. This technique consists in cloning a short fragment of a gene of interest into a viral vector, such as TRV (Tobacco rattle virus), and this viral construction is used to agro-infiltrate the plant. VIGS induces posttranscriptional gene silencing (PTGS) that results in the specific sequence degradation of target RNAs. Here we describe a VIGS protocol using the Gateway-based TRV vector for the study of genes in chili pepper plants.

Keywords: Capsicum; Chili pepper; Gene silencing; TRV; VIGS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Capsicum / genetics*
Gene Expression Regulation, Plant / genetics
Gene Silencing / physiology*
Genetic Vectors / genetics
Nicotiana / genetics*
Plant Viruses / genetics
Plant Viruses / pathogenicity
RNA Interference / physiology

Supplementary concepts

Tobacco rattle virus