Detoxification of aflatoxin B1 in corn by chlorine dioxide gas

Yang Yu; Jianyang Shi; Bingying Xie; Yutong He; Yongping Qin; Dan Wang; Haichun Shi; Yongpei Ke; Qun Sun

doi:10.1016/j.foodchem.2020.127121

Detoxification of aflatoxin B₁ in corn by chlorine dioxide gas

Food Chem. 2020 Oct 30:328:127121. doi: 10.1016/j.foodchem.2020.127121. Epub 2020 May 22.

Authors

Yang Yu¹, Jianyang Shi², Bingying Xie³, Yutong He⁴, Yongping Qin⁵, Dan Wang⁶, Haichun Shi⁷, Yongpei Ke⁷, Qun Sun⁸

Affiliations

¹ Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, PR China.
² Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, PR China; China Tobacco Sichuan Industry Co., Ltd., Chengdu 610066, PR China.
³ Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, PR China. Electronic address: 2017141241157@stu.scu.edu.cn.
⁴ Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, PR China. Electronic address: 2017141241144@stu.scu.edu.cn.
⁵ Institute of Drug Clinical Trials, West China Hospital, Sichuan University, Chengdu 610041, PR China.
⁶ College of Life Sciences, Chengdu Medical College, Chengdu, Sichuan 610500, PR China. Electronic address: wangdan@cmc.edu.cn.
⁷ Agronomy College, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
⁸ Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, PR China. Electronic address: qunsun@scu.edu.cn.

PMID: 32474241
DOI: 10.1016/j.foodchem.2020.127121

Abstract

Chlorine dioxide (ClO₂) gas was utilized for detoxifying aflatoxin B₁ (AFB₁) in corn for the first time. Four degradation compounds were identified by LC-MS as C₁₇H₁₃O₈, C₁₇H₁₅O₁₀, C₁₆H₁₅O₁₀, and C₁₅H₁₁O₈. Structurally, the biological activity of ClO₂-treated AFB₁ was removed due to the disappearance of C8-C9 double bond in the furan ring and the modification of cyclopentanone and methoxy after ClO₂ treatment. The cell viability assay on human embryo hepatocytes confirmed little toxicity of the degradation products. The degradation efficiency of AFB₁ on corn peaked near 90.0% under the optimized conditions and reached 79.6% for low initial contamination of AFB₁ at 5-20 μg/kg. Accordingly, ClO₂ has the potential to be developed into an effective, efficient, and economic approach to detoxify AFB₁ in grains.

Keywords: Aflatoxin B(1); ClO(2); Corn; Cytotoxicity; Detoxification; LC-MS; Mechanism.

MeSH terms

Aflatoxin B1 / chemistry*
Chlorine / chemistry*
Chlorine Compounds / chemistry*
Chromatography, High Pressure Liquid
Mass Spectrometry
Oxides / chemistry*
Zea mays / chemistry*

Substances

Chlorine Compounds
Oxides
Chlorine
chlorine dioxide
Aflatoxin B1