Biodiesel production from transesterification of Serratia sp. ISTD04 lipids using immobilised lipase on biocomposite materials of biomineralized products of carbon dioxide sequestrating bacterium

Production of biodiesel from lipids of Serratia sp. ISTD04 by lipase of Pseudomonas sp. ISTPL3 immobilised on biocomposite materials to increase the enzyme stability and reusability was studied. Lipase extracted, partially purifiedand immobilized onto activated biochar, impregnated with calcite obtained from biomineralization-based conversion of CO₂ from ISTD04, and bioactive ceramics materials, Na₂Ca₂Si₃O₉ prepared by chemical process. The composition, structure and texture of biocomposite materials determined by SEM and EDS methods. The composition of synthesized biodiesel was determined by GC-MS. The results imply that the immobilized lipase on activated biochar impregnated with calcite gave the maximum yield of fatty acid methyl esters (FAME:97.41%) followed by immobilized lipase on biochar (FAME:94.91), immobilized lipase on glass-ceramic (FAME:91.50%) and NaOH (FAME:85.63%). The reusability of lipase immobilized on activated biochar impregnated with calcite retained 75.11%and 50% catalytic activity after 5 and 10 cycles of transesterification reaction, respectively.