Partial nitritation performance and microbial community in sequencing batch biofilm reactor filled with zeolite under organics oppression and its recovery strategy

Ruixin Wang; Xiaojun Wang; Cuilan Deng; Zhenguo Chen; Yongxing Chen; Xinghui Feng; Zhong Zhong

doi:10.1016/j.biortech.2020.123031

Partial nitritation performance and microbial community in sequencing batch biofilm reactor filled with zeolite under organics oppression and its recovery strategy

Bioresour Technol. 2020 Jun:305:123031. doi: 10.1016/j.biortech.2020.123031. Epub 2020 Feb 19.

Authors

Ruixin Wang¹, Xiaojun Wang², Cuilan Deng¹, Zhenguo Chen³, Yongxing Chen¹, Xinghui Feng⁴, Zhong Zhong¹

Affiliations

¹ School of Environment and Energy, South China University of Technology, Guangzhou 510006, China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, China.
² School of Environment and Energy, South China University of Technology, Guangzhou 510006, China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, China; Hua An Biotech Co., Ltd., Foshan 528300, China. Electronic address: cexjwang@scut.edu.cn.
³ School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, China; Hua An Biotech Co., Ltd., Foshan 528300, China.
⁴ School of Environment and Energy, South China University of Technology, Guangzhou 510006, China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, China; Hua An Biotech Co., Ltd., Foshan 528300, China.

PMID: 32126482
DOI: 10.1016/j.biortech.2020.123031

Abstract

Influences of organics on partial nitritation performance were investigated in a lab-scale sequencing batch biofilm reactor filled with zeolite. Significant differences in nitrite production rate (NPR) were observed between different dosages of glucose. With influent COD/N ratio from 0 to 1.5, NPR declined from 0.4 to 0.05 kg/(m³·d). Meanwhile, an appropriate NO₂^--N/NH₄⁺-N ratio (1.4 ± 0.5) could be obtained for simultaneous anammox denitrification at COD/N ratio of 0.5. Increasing airflow rate was found as an effective recovery strategy. Other than competition of heterotrophs with nitrifiers for dissolved oxygen, it has been verified that addition of organics generated higher free ammonia, and then further inhibitedammonium oxidizing bacteria (AOB). Moreover, three-dimensional excitation-emission matrix (3D-EEM) results revealed that protein-like and humic acid-like substances were the main components in extracellularpolymericsubstances (EPS). And high-throughput sequencing analysis demonstrated that the relative abundance of AOB decreased.

Keywords: Free ammonia; Nitrosomonas; Organics; Partial nitritation; Zeolite.