Effect of different operational modes on the performance of granular sludge in continuous-flow systems and the successions of microbial communities

Shuai Li; Dong Li; Xuesong Ye; Shirui Zhang; Huiping Zeng; Yixing Yuan; Jie Zhang

doi:10.1016/j.biortech.2019.122573

Effect of different operational modes on the performance of granular sludge in continuous-flow systems and the successions of microbial communities

Bioresour Technol. 2020 Mar:299:122573. doi: 10.1016/j.biortech.2019.122573. Epub 2019 Dec 10.

Authors

Shuai Li¹, Dong Li², Xuesong Ye¹, Shirui Zhang², Huiping Zeng², Yixing Yuan³, Jie Zhang⁴

Affiliations

¹ State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
² Key Laboratory of Water Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100123, China.
³ State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address: yuan_yxing@163.com.
⁴ State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; Key Laboratory of Water Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100123, China.

PMID: 31865158
DOI: 10.1016/j.biortech.2019.122573

Abstract

Continuous flow reactors with time intermittent operational (TIO) mode and spatial intermittent operational (SIO) mode were operated to evaluate the effects of operational modes on the removal performances, the characteristics of granules and the dynamics of microbial communities in simultaneous nitrification, denitrification and phosphorus removal (SNDPR) granular system. The results showed that the removal efficiency of TP, TN were 81.3%, 86.7% under TIO mode, and 70.6%, 77.4% under SIO mode, respectively. Meanwhile, the PN and value of PN/PS in SIO were higher than those in TIO. Besides, results of high-throughput pyrosequencing illustrated that the combination of filamentous archaea (Methanothrix) and filamentous bacteria (Thiothrix) had resulted in the increase of EPS and SVI under SIO mode. Finally, functional bacterial and archaeal species, involving HMA, AMA, AOA, DPAOs etc., were identified to reveal the effects of operational modes on the mechanism of nutrients removal in granular SNDPR continuous-flow system.

Keywords: Continuous flow; High-throughput pyrosequencing; SNDPR granules; Spatial intermittent operational mode; Time intermittent operational mode.

MeSH terms

Bioreactors
Denitrification
Microbiota*
Nitrification
Nitrogen
Phosphorus
Sewage*
Waste Disposal, Fluid

Substances

Sewage
Phosphorus
Nitrogen