Ratiometric two-photon fluorescent probe for in situ imaging of carboxylesterase (CE)-mediated mitochondrial acidification during medication

Ao Jiang; Guang Chen; Jie Xu; Yuxia Liu; Guanghui Zhao; Zhenjun Liu; Tao Chen; Yulin Li; Tony D James

doi:10.1039/c9cc05759e

Ratiometric two-photon fluorescent probe for in situ imaging of carboxylesterase (CE)-mediated mitochondrial acidification during medication

Chem Commun (Camb). 2019 Sep 19;55(76):11358-11361. doi: 10.1039/c9cc05759e.

Authors

Ao Jiang¹, Guang Chen, Jie Xu, Yuxia Liu, Guanghui Zhao, Zhenjun Liu, Tao Chen, Yulin Li, Tony D James

Affiliation

¹ The Key Laboratory of Life-Organic Analysis, Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering, Qufu Normal University, The school attached to Qufu Normal University, Qufu 273165, China. chenandguang@163.com.

PMID: 31482158
DOI: 10.1039/c9cc05759e

Abstract

We report on a dual ratiometric two-photon fluorescent probe for in situ sensing of mitochondrial CE activity and pH. Using the probe it is possible to visualize the CE-mediated acidification of hepatoma cells and hepatic tissues during medication with antipyretic anti-inflammatory drugs.

MeSH terms

Anti-Inflammatory Agents, Non-Steroidal / pharmacology
Antipyretics / pharmacology
Carboxylesterase / analysis*
Carboxylesterase / metabolism
Cell Proliferation / drug effects
Fluorescent Dyes / chemistry*
Hep G2 Cells
Humans
Hydrogen-Ion Concentration
Liver / drug effects
Liver / metabolism
Mitochondria / drug effects
Mitochondria / metabolism*
Optical Imaging*
Photons*

Substances

Anti-Inflammatory Agents, Non-Steroidal
Antipyretics
Fluorescent Dyes
Carboxylesterase