Herbal medicine against genotoxicity of dimethoate, an insecticide, in mammalian somatic cells

Halit Kızılet; Bilal Yilmaz; Handan Uysal

doi:10.1016/j.heliyon.2019.e01337

Herbal medicine against genotoxicity of dimethoate, an insecticide, in mammalian somatic cells

Heliyon. 2019 Mar 16;5(3):e01337. doi: 10.1016/j.heliyon.2019.e01337. eCollection 2019 Mar.

Authors

Halit Kızılet¹, Bilal Yilmaz², Handan Uysal³

Affiliations

¹ Department of Cardiology, Erzurum Regional Training and Research Hospital, 25100, Erzurum, Turkey.
² Department of Analytical Chemistry, Faculty of Pharmacy, Atatürk University, 25240, Erzurum, Turkey.
³ Department of Biology, Faculty of Science, Atatürk University, 25240, Erzurum, Turkey.

Abstract

In this study, the genotoxic effects of dimethoate (DIM) were investigated with the in vitro micronucleus test in human peripheral lymphocytes. The ethanol extracts of Rosa canina and Salvia lavandulifolia were used to remove possible genotoxic effects of these substances. For this purpose, different concentrations (0.5-1-2 μg/mL) of dimethoate, DIM + RC_eta and DIM + SL_eta (1:1 v/v) application groups were prepared and applied to the blood culture. The obtained data were compared with the negative control group that was prepared with dimethyl sulfoxide (DMSO) as solvent and a well-known genotoxic effects of ethyl methanesulfonate (EMS) as positive control group. It was observed in lymphocyte cells that the frequency of MN considerably increased depending on the increasing dose of DIM whereas the nuclear division index (NBI)decreased according to the control group, especially in the last concentration (2 μg/mL). But, as the MN frequency decreased, NBI values approached to control group with 2μg/mL DIM + RC_eta and 2μg/mL DIM + SL_eta according to DIM application group (P < 0.05). Additionally, RC_eta and SL_eta were analyzed by gas chromotography-mass spectrometry (GC-MS).

Keywords: Genetics; Toxicology.