Achyranthes bidentata polypeptides promotes migration of Schwann cells via NOX4/DUOX2-dependent ROS production in rats

Honghua Song; Hualong Zhao; Lingyan Yang; Lilan Li; Tuanjie Zhang; Jiacheng Pan; Yali Meng; Weixing Shen; Ying Yuan

doi:10.1016/j.neulet.2018.12.023

Achyranthes bidentata polypeptides promotes migration of Schwann cells via NOX4/DUOX2-dependent ROS production in rats

Neurosci Lett. 2019 Mar 23:696:99-107. doi: 10.1016/j.neulet.2018.12.023. Epub 2018 Dec 17.

Authors

Honghua Song¹, Hualong Zhao², Lingyan Yang³, Lilan Li⁴, Tuanjie Zhang⁵, Jiacheng Pan⁶, Yali Meng⁷, Weixing Shen⁸, Ying Yuan⁹

Affiliations

¹ Department of Pediatrics, Affiliated Hospital of Nantong University, Nantong, 226001, PR China. Electronic address: song_hong_hua@126.com.
² Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, PR China. Electronic address: zhaohualong2011@163.com.
³ Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, PR China. Electronic address: 124152828@qq.com.
⁴ Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, PR China. Electronic address: 1152280227@qq.com.
⁵ Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, PR China. Electronic address: 383761825@qq.com.
⁶ Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, PR China. Electronic address: kaixinge@126.com.
⁷ Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, PR China. Electronic address: 965048703@qq.com.
⁸ Medical School, Nantong University, Nantong, 226001, PR China. Electronic address: swx@ntu.edu.cn.
⁹ Department of Personnel, Affiliated Hospital of Nantong University, Nantong, 226001, PR China; Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, PR China. Electronic address: yuanying@ntu.edu.cn.

PMID: 30572102
DOI: 10.1016/j.neulet.2018.12.023

Abstract

Achyranthes bidentata polypeptides (ABPP), an active polypeptides isolated from the aqueous extract of Achyranthes bidentata Blume, contributes to the regeneration of injured peripheral nerves by promoting migration of Schwann cells (SCs). In this study, we aimed to investigate the possible mechanism underlying the ABPP-induced migration of primary cultured rat SCs. Transwell migration assays indicated that ABPP promoted SCs migration in a concentration-dependent manner by inducing production of NADPH-oxidase (NOX)-derived reactive oxygen species (ROS). Inhibition of ROS production by NOXs inhibitor apocynin (APO) or diphenyleneiodonium (DPI) partially blocked ABPP-mediated SCs migration. Furthermore, by using real-time polymerase chain reaction analysis and siRNA interference technique, we verified the participation of NOX subunit 4 (NOX4) and dual oxidase 2 (DUOX2) in ABPP-induced ROS production and consequential SCs migration. Taken together, these results demonstrated that ABPP promoted SCs migration via NOX4/DUOX2-activated ROS in SCs.

Keywords: ABPP; DUOX2; NOX4; ROS; Schwann cells migration.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Achyranthes / metabolism
Animals
Dual Oxidases / metabolism
NADPH Oxidase 4 / metabolism
Neurons / drug effects
Neuroprotective Agents / pharmacology*
Peptides / pharmacology*
Plant Extracts / pharmacology
Rats, Sprague-Dawley
Reactive Oxygen Species / metabolism*
Schwann Cells / drug effects*

Substances

Neuroprotective Agents
Peptides
Plant Extracts
Reactive Oxygen Species
Dual Oxidases
NADPH Oxidase 4
Nox4 protein, rat