Short-term in vitro culture of purity and highly functional rat bone marrow-derived mast cells

In Vitro Cell Dev Biol Anim. 2018 Dec;54(10):705-714. doi: 10.1007/s11626-018-0301-3. Epub 2018 Oct 19.

Abstract

Mast cells (MCs) are responsible for the innate immune response. Rat MCs are more suitable than mouse MCs as models of specific parasite infection processes and ovalbumin-induced asthma. Rat peritoneum-derived MCs and RBL-2H3 cells (an MC cell line) are widely used in disease studies. However, the application of rat bone marrow-derived MCs (BMMCs) are poorly documented in terms of the methodology of rat BMMC isolation. Here, we describe a relatively rapid, efficient, and simple method for the cultivation of rat BMMCs. As compared to previous protocols, rat BMMCs produced with the proposed protocol exhibited advantages in differentiation, proliferation, lifespan, and functionality, which should prove useful for studies of mucosal MC diseases in specific rat models.

Keywords: AA4; Bone marrow-derived mast cell; Differentiation; FcεRI; In vitro; Purification; Rat.

MeSH terms

  • Animals
  • Bone Marrow Cells / cytology*
  • Cell Culture Techniques / methods*
  • Cell Degranulation
  • Cell Separation
  • Cells, Cultured
  • Chymases / genetics
  • Chymases / metabolism
  • Histamine / metabolism
  • Male
  • Mast Cells / cytology*
  • Mast Cells / metabolism
  • Peptide Hydrolases / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats, Sprague-Dawley
  • beta-N-Acetylhexosaminidases / metabolism

Substances

  • RNA, Messenger
  • Histamine
  • beta-N-Acetylhexosaminidases
  • Peptide Hydrolases
  • chymase 2
  • Chymases
  • Mcpt1 protein, rat