Iron depletion alters surface-associated properties of Staphylococcus aureus and its association to human neutrophils in chemiluminescence

P A Domingue; P A Lambert; M R Brown

doi:10.1016/0378-1097(89)90429-1

Iron depletion alters surface-associated properties of Staphylococcus aureus and its association to human neutrophils in chemiluminescence

FEMS Microbiol Lett. 1989 Jun;50(3):265-8. doi: 10.1016/0378-1097(89)90429-1.

Authors

P A Domingue¹, P A Lambert, M R Brown

Affiliation

¹ Department of Pharmaceutical Sciences, Aston University, Birmingham, U.K.

PMID: 2759418
DOI: 10.1016/0378-1097(89)90429-1

Abstract

Staphylococcus aureus NCTC 6571 was grown in iron-depleted tryptone soya broth (Fe-TSB) to approximate to in vivo conditions, and in iron-rich TSB (Fe + TSB). Low iron effected a crucial decrease in surface hydrophobicity (SH) and a lack of supernatant Protein A (PrA). Iron availability did not affect PrA detection in immunoblotting and it was identified as a 35.5 kDa antigen in this strain. Fe-phenotypes lacked 34, 48 and 52 kDa antigens. In chemiluminescence, Fe-phenotypes appeared least vulnerable to phagocytosis despite opsonisation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens, Surface
Cell Membrane / drug effects
Cell Membrane / immunology
Cell Membrane / metabolism
Humans
In Vitro Techniques
Iron / pharmacology*
Luminescent Measurements
Neutrophils / immunology*
Phagocytosis / drug effects
Staphylococcal Protein A / metabolism
Staphylococcus aureus / drug effects*
Staphylococcus aureus / immunology
Staphylococcus aureus / metabolism

Substances

Antigens, Surface
Staphylococcal Protein A
Iron