In vitro and in vivo hemocompatibility evaluation of a new dermatan sulfate-modified PET patch for vascular repair surgery

J Biomed Mater Res B Appl Biomater. 2017 Oct;105(7):2001-2009. doi: 10.1002/jbm.b.33733. Epub 2016 Jul 1.

Abstract

The development of new vascular devices requires to study the effects of materials on blood cells and on coagulation, both in vitro and in vivo. In this study, we have developed a new material by grafting dermatan sulfate (DS) from shark skin onto polyethylene terephthalate (PET). We have evaluated the haemocompatibility of PET-DS material in vitro by measuring thrombin generation, plasma recalcification time, hemolytic activity, and platelet adhesion and in vivo with a model of vascular patch in rat abdominal aorta. In vitro, our results have shown that PET-DS is a nonhemolytic material, able to inhibit thrombin generation and platelet adhesion. In vivo studies by Doppler echographic evaluation 20 days after implantation have shown that the PET-DS patch was integrated in the vessel wall and covered by a layer of cells. In conclusion, PET-DS has good haemocompatibility properties and could be a promising tool for vascular surgery. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2001-2009, 2017.

Keywords: dermatan sulfate; haemocompatibility; immobilization; in vitro; in vivo; material testing; polyethylene terephthalate (PET); vascular patch.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aorta, Abdominal / metabolism
  • Aorta, Abdominal / pathology
  • Aorta, Abdominal / surgery*
  • Blood Platelets / metabolism*
  • Dermatan Sulfate / chemistry
  • Dermatan Sulfate / pharmacology*
  • Humans
  • Male
  • Materials Testing*
  • Platelet Adhesiveness / drug effects*
  • Rats
  • Rats, Wistar
  • Sharks*
  • Skin / chemistry*

Substances

  • Dermatan Sulfate