Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues

Carbohydr Res. 2016 Apr 7:424:54-8. doi: 10.1016/j.carres.2016.02.006. Epub 2016 Feb 24.

Abstract

The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain was extracted from specific tissues of several kinds of sharks and rays. The contents and sulfation patterns of the CS/DS hybrid chain were precisely analyzed by digestion with chondroitinases ABC and AC. All samples predominantly contained the A- and C-units. Furthermore, all samples characteristically contained the D-unit. Species-specific differences were observed in the contents of the CS/DS hybrid chain, which were the highest in Mako and Blue sharks and Sharpspine skates, but were lower in Hammerhead sharks. Marked differences were observed in the ratio of the C-unit/A-unit between sharks and rays. The contents of the CS/DS hybrid chain and the ratio of the C-unit/A-unit may be related to an oxidative stress-decreasing ability.

Keywords: Chondroitin sulfate; Dermatan sulfate; Glycosaminoglycan; Ray; Shark.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chondroitin ABC Lyase / chemistry
  • Chondroitin Lyases / chemistry
  • Chondroitin Sulfates / chemistry*
  • Dermatan Sulfate / chemistry*
  • Oxidative Stress*
  • Sharks
  • Skates, Fish

Substances

  • Dermatan Sulfate
  • Chondroitin Sulfates
  • Chondroitin Lyases
  • Chondroitin ABC Lyase