Objective: To observe the effects of rosiglitazone (ROSI) on endotoxin-induced acute liver injury in rats.
Methods: All the animals were randomly assigned to one of four following groups (n=24 per group). Rats in the Sepsis-Dimethyl sulfoxide (DMSO) group(n=24), rats were treated with 10%DMSO(1 ml/kg) 30 mins before they were challenged with cecal ligation and puncture(CLP) (n=24). In the sepsis-rosiglitazone (ROSI) group was identical to the sepsis-DMSO group but received ROSI (10 mg/kg intravenously) (n=24). In the sham-DMSO group, rats were treated with 10% DMSO(1 ml/kg) 30 mins before the administration of saline (n=24). The sham-ROSI group was identical to the sham-ROSI group except that ROSI (10 mg/kg intravenously) was administered instead of DMSO (n=24). Sepsis was induced in rats by dissecting the cecum and placing the discontinued organ back into the abdomen CLP. And all the Rats were decapitated at 0, 6, 12, 24 h after the model completed.Serum glutamic oxaloacetic transaminase(GOT), glutamic pyruvic transaminase (GPT), interleukin 12 (IL-12) were determined to assess activity and liver pathologic change of rats in different groups. Data were analyzed with SPSS 19.0 software.Student's t-test was used to compare densitometry data.
Results: SPSS 19.0 statistical software analysis by the outcome of the application ELASA method for determining zero hours between every group, there was non-difference. The sepsis-ROSI group and the sepsis-DMSO group 12 hours and 24 hours the presence of plasma GOT concentration changes statistically significant difference (t12=3.97, P12=0.01; t24=3.10, P24<0.001), (t12=0.55, P12<0.001; t24=4.32, P24<0.001). The Sepsis-ROSI group and the sepsis-DMSO group 6 hours, 12 hours and 24 hours the presence of plasma GPT concentration changes statistically significant difference (t6=3.17, P6=0.021; t12=2.81, P12=0.018; t24=5.06, P24<0.001), (t6=2.67, P6=0.001; t12=3.36, P12=0.002; t24=2.83, P24<0.001). The sepsis-ROSI group and the sepsis-DMSO group 6 hours, 12 hours and 24 hours the presence of plasma IL-12 concentration changes statistically significant difference(t6=3.17, P6=0.021; t12=2.81, P12=0.018; t24=5.06, P24<0.001), (t6=2.67, P6=0.001; t12=3.36, P12=0.002; t24=2.83, P24<0.001). Sepsis GOT, GPT, IL-12 began to increase in 6 hours, 24 hours of high-value, no clear peak trend, the sepsis-ROSI group compared with the sham-ROSI group had lower concentrations of GOT, GPT and IL-12 to some extent. Description rosiglitazone inhibit inflammatory cytokines in rats with sepsis role of IL-12, there is a certain role to reduce the inflammatory response in sepsis.
Conclusions: Treatment with Thiazolidinedione, markedly inhibited the expression of IL-12, attenuated pulmonary inflammation and liver injury caused by sepsis in rat.