Determination of L-thyroxine in pharmaceutical preparations by flow injection analysis with chemiluminescence detection based on the enhancement of the luminol-KMnO4 reaction in a micellar medium

Spectrochim Acta A Mol Biomol Spectrosc. 2015 Apr 5:140:162-5. doi: 10.1016/j.saa.2014.12.105. Epub 2015 Jan 5.

Abstract

A novel flow injection chemiluminescence (CL) method for the determination of L-thyroxine in the presence of cethyltrimethylammonium bromide (CTMAB) surfactant micelles is developed. The method is based on the significant signal enhancement of L-thyroxine on the luminol-KMnO4 system in alkaline solution sensitized by CTMAB. Parameters affecting the reproducibility and CL detection were optimized systematically. Under the optimum conditions, the net CL intensity versus L-thyroxine concentration was linear in the range of 5.0×10(-8)-3.0×10(-6) mol/L with the detection limit of 8.9×10(-9) mol/L. The sample throughput is calculated to be 140 samples/h and the relative standard deviations (RSDs) for 13 replicate determination of 1.0×10(-6) L-thyroxine is 1.1%. The proposed method was successfully applied for the determination of L-thyroxine in pharmaceutical preparations with satisfactory recoveries in the range of 93.9-105.2%. This rapid, sensitive, and high throughput method would provide a new tool for L-thyroxine analysis.

Keywords: Cethyltrimethylammonium bromide; Chemiluminescence; Flow-injection; Luminol; l-thyroxine.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Equipment Design
  • Flow Injection Analysis / instrumentation*
  • Limit of Detection
  • Luminescence
  • Luminescent Measurements / instrumentation*
  • Luminol / chemistry*
  • Micelles
  • Potassium Permanganate / chemistry*
  • Reproducibility of Results
  • Surface-Active Agents / chemistry
  • Thyroxine / analysis*

Substances

  • Micelles
  • Surface-Active Agents
  • Potassium Permanganate
  • Luminol
  • Thyroxine