Protein engineering of cellulases

Andreas S Bommarius; Minjeong Sohn; Yuzhi Kang; Jay H Lee; Matthew J Realff

doi:10.1016/j.copbio.2014.04.007

Protein engineering of cellulases

Curr Opin Biotechnol. 2014 Oct:29:139-45. doi: 10.1016/j.copbio.2014.04.007. Epub 2014 May 8.

Authors

Andreas S Bommarius¹, Minjeong Sohn², Yuzhi Kang³, Jay H Lee⁴, Matthew J Realff⁵

Affiliations

¹ School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA; Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA; School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA. Electronic address: andreas.bommarius@chbe.gatech.edu.
² School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA; Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA; School of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon, South Korea.
³ School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA; Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA.
⁴ School of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon, South Korea. Electronic address: jayhlee@kaist.ac.kr.
⁵ School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.

PMID: 24794535
DOI: 10.1016/j.copbio.2014.04.007

Abstract

This review covers the topic of protein engineering of cellulases, mostly after 2009. Two major trends that are identified in this work are: first, the increased importance of results from computational protein engineering to drive ideas in the field, as experimental ideas and results often are still scarce, and, second, the further development of helper proteins for cellulose hydrolysis, such as lytic polysaccharide monooxygenase (LPO). The discussion in this work focuses both on improved attributes of cellulases and on the domains of cellulase that have been improved.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Biocatalysis
Cellulases / metabolism*
Cellulose / metabolism
Enzyme Stability
Glycosylation
Protein Engineering / methods*

Substances

Cellulose
Cellulases