Toxoplasma gondii induce apoptosis of neural stem cells via endoplasmic reticulum stress pathway

Parasitology. 2014 Jun;141(7):988-95. doi: 10.1017/S0031182014000183. Epub 2014 Mar 10.

Abstract

Toxoplasma gondii is a major cause of congenital brain disease; however, the underlying mechanism of neuropathogenesis in brain toxoplasmosis remains elusive. To explore the role of T. gondii in the development of neural stem cells (NSCs), NSCs were isolated from GD14 embryos of ICR mice and were co-cultured with tachyzoites of T. gondii RH strain. We found that apoptosis levels of the NSCs co-cultured with 1×106 RH tachyzoites for 24 and 48 h significantly increased in a dose-dependent manner, as compared with the control. Western blotting analysis displayed that the protein level of C/EBP homologous protein (CHOP) was up-regulated, and caspase-12 and c-Jun N-terminal kinase (JNK) were activated in the NSCs co-cultured with the parasites. Pretreatment with endoplasmic reticulum stress (ERS) inhibitor (TUDCA) and caspase-12 inhibitor (Z-ATAD-FMK) inhibited the expression or activation of the key molecules involved in the ERS-mediated apoptotic pathway, and subsequently decreased the apoptosis levels of the NSCs induced by the T. gondii. The findings here highlight that T. gondii induced apoptosis of the NSCs through the ERS signal pathway via activation of CHOP, caspase-12 and JNK, which may constitute a potential molecular mechanism responsible for the cognitive disturbance in neurological disorders of T. gondii.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis Regulatory Proteins / physiology*
  • Coculture Techniques
  • Embryo, Mammalian
  • Endoplasmic Reticulum / physiology*
  • Female
  • Male
  • Mice
  • Mice, Inbred ICR
  • Mitochondrial Proteins / physiology*
  • Neural Stem Cells / physiology*
  • Stress, Physiological
  • Toxoplasma / physiology*

Substances

  • Apopt1 protein, mouse
  • Apoptosis Regulatory Proteins
  • Mitochondrial Proteins