Let7a inhibits the growth of endometrial carcinoma cells by targeting Aurora-B

FEBS Lett. 2013 Aug 19;587(16):2523-9. doi: 10.1016/j.febslet.2013.05.065. Epub 2013 Jun 12.

Abstract

MicroRNAs negatively regulate target gene expression at the post-transcriptional level during carcinogenesis. Recent advances revealed that the expression levels of several miRNAs are up- or down-regulated in endometrial carcinoma (EC). Here we identify dysregulated miRNAs in EC and we elucidate the essential role of let-7a. The expression of 86 miRNAs in EC was found to be different from adjacent normal endometrial tissues. Moreover, miR-let-7 members are down-regulated in EC and let-7 miRNAs are highly associated with endometrial cancer. A functional investigation revealed that let-7a suppressed proliferation of HeLa cells by targeting Aurora-B. Let-7a also antagonizes Aurora-B functions in promoting carcinoma cell proliferation by down-regulating Aurora-B protein level. Let-7a could be applied for gene therapy against endometrial carcinogenesis.

Keywords: Endometrial carcinoma cell; Let-7a; MicroRNA; Targeting Aurora-B.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aurora Kinase B
  • Aurora Kinases
  • Cell Proliferation
  • Cell Survival
  • Endometrial Neoplasms / genetics
  • Endometrial Neoplasms / pathology*
  • Endometrium / enzymology*
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Enzymologic*
  • Gene Expression Regulation, Neoplastic*
  • HeLa Cells
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / physiology*
  • Oligonucleotide Array Sequence Analysis
  • Protein Serine-Threonine Kinases / metabolism*

Substances

  • MicroRNAs
  • mirnlet7 microRNA, human
  • AURKB protein, human
  • Aurora Kinase B
  • Aurora Kinases
  • Protein Serine-Threonine Kinases