ABC-cassette transporter 1 (ABCA1) expression in epithelial cells in Chlamydia pneumoniae infection

Microb Pathog. 2013 Aug-Sep:61-62:57-61. doi: 10.1016/j.micpath.2013.05.006. Epub 2013 May 21.

Abstract

ATP-binding cassette transporter A1 (ABCA1) mediates reverse cholesterol transport and innate immunity response in different cell types. We have investigated the regulation of ABCA1 expression in response to intracellular Chlamydia pneumoniae infection in A549 epithelial lung carcinoma cells. C. pneumoniae infection decreased ABCA1 expression in A549 cells, and the activity of the ABCA1 promoter was decreased. The decreased promoter activity was dependent on its E-box and GnT-box elements of the promoter. Chlamydial growth was decreased in ABCA1-silenced epithelial lung carcinoma cells. These data indicate an important role for ABCA1 in intracellular bacterial infection.

Keywords: ABCA1; Chlamydia pneumoniae; Epithelial cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter 1 / genetics
  • ATP Binding Cassette Transporter 1 / metabolism*
  • Cell Line, Tumor
  • Chlamydophila pneumoniae / pathogenicity*
  • Epithelial Cells / metabolism*
  • Epithelial Cells / microbiology
  • Gene Expression Regulation
  • Humans
  • Lung / cytology
  • Lung / microbiology
  • Promoter Regions, Genetic
  • Transcription, Genetic

Substances

  • ABCA1 protein, human
  • ATP Binding Cassette Transporter 1