Effective suppression of azoxymethane-induced aberrant crypt foci formation in mice with citrus peel flavonoids

Ching-Shu Lai; Shiming Li; Cheng Bin Liu; Yutaka Miyauchi; Michiko Suzawa; Chi-Tang Ho; Min-Hsiung Pan

doi:10.1002/mnfr.201200606

Effective suppression of azoxymethane-induced aberrant crypt foci formation in mice with citrus peel flavonoids

Mol Nutr Food Res. 2013 Mar;57(3):551-5. doi: 10.1002/mnfr.201200606. Epub 2013 Jan 10.

Authors

Ching-Shu Lai¹, Shiming Li, Cheng Bin Liu, Yutaka Miyauchi, Michiko Suzawa, Chi-Tang Ho, Min-Hsiung Pan

Affiliation

¹ Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung, Taiwan.

PMID: 23307625
DOI: 10.1002/mnfr.201200606

Abstract

Citrus peel or its extract has been reported to exhibit a broad spectrum of biological activity. Herein, we report the first investigation of inhibitory effects of a formulated product from citrus peel extract, gold lotion (GL), on azoxymethane-induced colonic tumorigenesis. We have demonstrated that oral feeding of GL decreased the number of aberrant crypt foci (ACF), particularly large size of ACF in colonic tissues of mice. Both gene and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were suppressed by GL treatment. The in vivo data have revealed for the first time that the citrus peel extract-GL-is an effective antitumor agent mechanistically downregulating the protein levels of iNOS, COX-2, ornithine decarboxylase, vascular endothelial growth factor, and matrix metallopeptidase 9 in colonic tissues of mice, suggesting that GL is a novel functional natural product capable of preventing inflammation-associated colon tumorigenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aberrant Crypt Foci / chemically induced
Aberrant Crypt Foci / drug therapy*
Aberrant Crypt Foci / pathology
Administration, Oral
Animals
Antineoplastic Agents, Phytogenic / pharmacology*
Azoxymethane / toxicity*
Citrus / chemistry*
Colonic Neoplasms / chemically induced
Colonic Neoplasms / drug therapy*
Colonic Neoplasms / pathology
Cyclooxygenase 2 / genetics
Cyclooxygenase 2 / metabolism
Flavonoids / administration & dosage
Flavonoids / pharmacology*
Male
Matrix Metalloproteinase 9 / metabolism
Mice
Mice, Inbred ICR
Nitric Oxide Synthase Type II / genetics
Nitric Oxide Synthase Type II / metabolism
Ornithine Decarboxylase / metabolism
Plant Extracts / pharmacology
Vascular Endothelial Growth Factor A / metabolism

Substances

Antineoplastic Agents, Phytogenic
Flavonoids
Plant Extracts
Vascular Endothelial Growth Factor A
vascular endothelial growth factor A, mouse
Nitric Oxide Synthase Type II
Nos2 protein, mouse
Ptgs2 protein, mouse
Cyclooxygenase 2
Matrix Metalloproteinase 9
Ornithine Decarboxylase
Azoxymethane