A novel variant, NDM-5, of the New Delhi metallo-β-lactamase in a multidrug-resistant Escherichia coli ST648 isolate recovered from a patient in the United Kingdom

Antimicrob Agents Chemother. 2011 Dec;55(12):5952-4. doi: 10.1128/AAC.05108-11. Epub 2011 Sep 19.

Abstract

A new variant of the New Delhi metallo-enzyme (NDM) carbapenemase was identified in a multidrug-resistant Escherichia coli ST648 isolate recovered from the perineum and throat of a patient in the United Kingdom with a recent history of hospitalization in India. NDM-5 differed from existing enzymes due to substitutions at positions 88 (Val → Leu) and 154 (Met → Leu) and reduced the susceptibility of E. coli TOP10 transformants to expanded-spectrum cephalosporins and carbapenems when expressed under its native promoter.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Amino Acid Substitution
  • Anti-Bacterial Agents / pharmacology
  • Carbapenems / pharmacology
  • Cephalosporins / pharmacology
  • Drug Resistance, Multiple, Bacterial / genetics*
  • Escherichia coli / drug effects*
  • Escherichia coli / enzymology
  • Escherichia coli / isolation & purification
  • Escherichia coli Infections / epidemiology
  • Escherichia coli Infections / microbiology
  • Genetic Variation*
  • Humans
  • India / epidemiology
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Sequence Analysis, DNA
  • United Kingdom
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • Carbapenems
  • Cephalosporins
  • beta-Lactamases
  • beta-lactamase NDM-1

Associated data

  • GENBANK/AEA41876
  • GENBANK/FN396876
  • GENBANK/JN104597