Abstract
A new variant of the New Delhi metallo-enzyme (NDM) carbapenemase was identified in a multidrug-resistant Escherichia coli ST648 isolate recovered from the perineum and throat of a patient in the United Kingdom with a recent history of hospitalization in India. NDM-5 differed from existing enzymes due to substitutions at positions 88 (Val → Leu) and 154 (Met → Leu) and reduced the susceptibility of E. coli TOP10 transformants to expanded-spectrum cephalosporins and carbapenems when expressed under its native promoter.
MeSH terms
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Adult
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Amino Acid Substitution
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Anti-Bacterial Agents / pharmacology
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Carbapenems / pharmacology
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Cephalosporins / pharmacology
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Drug Resistance, Multiple, Bacterial / genetics*
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Escherichia coli / drug effects*
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Escherichia coli / enzymology
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Escherichia coli / isolation & purification
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Escherichia coli Infections / epidemiology
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Escherichia coli Infections / microbiology
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Genetic Variation*
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Humans
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India / epidemiology
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Sequence Analysis, DNA
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United Kingdom
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beta-Lactamases / genetics*
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beta-Lactamases / metabolism
Substances
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Anti-Bacterial Agents
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Carbapenems
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Cephalosporins
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beta-Lactamases
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beta-lactamase NDM-1
Associated data
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GENBANK/AEA41876
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GENBANK/FN396876
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GENBANK/JN104597