An IRF-3 homolog that is up-regulated by DNA virus and poly I:C in turbot, Scophthalmus maximus

Fish Shellfish Immunol. 2011 Dec;31(6):1224-31. doi: 10.1016/j.fsi.2011.07.011. Epub 2011 Jul 19.

Abstract

In this study, we described the structure, mRNA tissue distribution and regulation of an IRF-3 gene from turbot, Scophthalmus maximus (SmIRF-3). The gene sequence of SmIRF-3 is 6077 bp long, composed of 11 exons and 10 introns similar to known IRF-3 genes of fish, and encodes a peptide of 466 amino acids. The deduced protein sequence shares the highest identity of 56.0-81.2% with fish IRF-3 and possesses a DNA-binding domain (DBD), an IRF association domain (IAD) and a serine-rich domain (SRD) known to be important for the functions of IRF-3 in vertebrates. Phylogenetic analysis grouped SmIRF-3 with other IRF3s of vertebrates. SmIRF-3 transcripts were detectable in limited tissue types of healthy fish, with higher expression observed in head, kidney, spleen and kidney,. The SmIRF-3 was transcriptionally up-regulated by turbot reddish body iridovirus (TRBIV) and polyinosinic:polycytidylic acid (poly I:C) in the head kidney, spleen and gills, with showing a two wave induced expression during a 7-day time course in all cases. The highest inducibility and the likely earliest increase of SmIRF-3 expression were observed in the spleen, and poly I:C was a stronger inducer. In addition, the maximal expression level of SmIRF-3 arose prior to that of the Mx in all the cases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers / genetics
  • Flatfishes / genetics*
  • Gene Components
  • Gene Expression Regulation / immunology*
  • Head Kidney / metabolism
  • Interferon Regulatory Factor-3 / genetics*
  • Interferon Regulatory Factor-3 / metabolism*
  • Iridovirus / immunology*
  • Kidney / metabolism
  • Molecular Sequence Data
  • Phylogeny
  • Poly I-C / immunology*
  • Protein Structure, Tertiary
  • RNA, Messenger / genetics*
  • Real-Time Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sequence Homology
  • Species Specificity
  • Spleen / metabolism

Substances

  • DNA Primers
  • Interferon Regulatory Factor-3
  • RNA, Messenger
  • Poly I-C