Berberine hydrochloride attenuates cyclooxygenase-2 expression in rat small intestinal mucosa during acute endotoxemia

Ai-Wen Feng; Chao Yu; Qing Mao; Ning Li; Qiu-Rong Li; Jie-Shou Li

doi:10.1016/j.fitote.2011.05.013

Berberine hydrochloride attenuates cyclooxygenase-2 expression in rat small intestinal mucosa during acute endotoxemia

Fitoterapia. 2011 Oct;82(7):976-82. doi: 10.1016/j.fitote.2011.05.013. Epub 2011 May 27.

Authors

Ai-Wen Feng¹, Chao Yu, Qing Mao, Ning Li, Qiu-Rong Li, Jie-Shou Li

Affiliation

¹ School of Medicine, Nanjing University, Nanjing, Jiangsu 210093, China.

PMID: 21641970
DOI: 10.1016/j.fitote.2011.05.013

Abstract

The effect of berberine hydrochloride (BBR) on inducible cyclooxygenase-2 (COX-2) in small intestinal mucosa and related mechanisms was investigated in a rat model of acute endotoxemia. The results showed that lipopolysaccharide (LPS) increased COX-2 expression, whereas SB202190 and BBR curtailed it. LPS increased phosphorylation of mucosal p38 MAPK and ATF2 as well as production of ATF2, whereas BBR attenuated these effects. LPS upregulated mucosal peroxisome proliferator-activated receptor gamma (PPARγ), but BBR reduced this receptor. GW9662 aggravated LPS-induced and reversed BBR-attenuated COX-2 expression. The findings showed that BBR ameliorated COX-2 overexpression partially via modulation of p38 and PPARγ pathways during acute endotoxemia.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acute Disease
Anilides
Animals
Anti-Inflammatory Agents / pharmacology*
Berberine / pharmacology*
Coptis / chemistry
Cyclooxygenase 2 / metabolism*
Cyclooxygenase 2 Inhibitors / pharmacology
Cyclooxygenase 2 Inhibitors / therapeutic use*
Disease Models, Animal
Endotoxemia / drug therapy*
Endotoxemia / metabolism
Enzyme Inhibitors / pharmacology
Imidazoles / pharmacology
Intestinal Mucosa / drug effects*
Intestinal Mucosa / metabolism
Intestine, Small / drug effects
Intestine, Small / metabolism
Lipopolysaccharides
Male
PPAR gamma / metabolism
Phosphorylation
Phytotherapy*
Plant Extracts / pharmacology
Plant Extracts / therapeutic use
Pyridines / pharmacology
Rats
Rats, Sprague-Dawley
Rhizome
Up-Regulation
p38 Mitogen-Activated Protein Kinases / metabolism

Substances

2-chloro-5-nitrobenzanilide
Anilides
Anti-Inflammatory Agents
Cyclooxygenase 2 Inhibitors
Enzyme Inhibitors
Imidazoles
Lipopolysaccharides
PPAR gamma
Plant Extracts
Pyridines
Berberine
Cyclooxygenase 2
p38 Mitogen-Activated Protein Kinases
4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl)imidazole