Type specificity and use of human sera for rapid typing of herpes simplex virus isolates by an indirect peroxidase-labeled antibody technique: a comparison with three other methods

Arch Virol. 1977;54(1-2):119-30. doi: 10.1007/BF01314384.

Abstract

Human sera from patients showing seroconversion following a primary herpes simplex virus type 1 (HSV-1) or HSV type 2 (HSV-2) infection, as well as 19S and 7S fractions obtained from the same sera, have been used for typing 191 HSV isolates by an indirect peroxidase-labeled antibody (PLA) method. Typing has also been performed on all of the isolates using a microneutralization (MN) and the indirect hemagglutination (IHA) inhibition test. Furthermore, 30 isolates have been typed by a plaque method. Results obtained with the PLA method was in complete agreement with those of the other two procedures. The PLA method is rapid and simple, offers easy interpretation and a permanent record of results.

Publication types

  • Comparative Study

MeSH terms

  • Antibody Specificity
  • Evaluation Studies as Topic
  • Hemagglutination Inhibition Tests
  • Herpes Simplex / microbiology*
  • Humans
  • Immune Sera
  • Immunoenzyme Techniques*
  • Immunoglobulin G
  • Immunoglobulin M
  • Neutralization Tests
  • Simplexvirus / classification*

Substances

  • Immune Sera
  • Immunoglobulin G
  • Immunoglobulin M