[Molecular classification of a phytoplasma associated with willow yellow disease]

Wei Sheng Wu Xue Bao. 2009 Mar;49(3):389-94.
[Article in Chinese]

Abstract

Objective: Willow Yellow is a plant disease caused by phytoplasma. We identified the taxonomic status of Willow Yellow phytoplasma (WY).

Methods: We used specific conservative primers amplified 16S rDNA and ribosomal proteins gene (rp) gene. Obtained sequences were then analyzed and homology trees were constructed. The nested-PCR products were also analyzed by restriction fragment length polymorphism (RFLP).

Results: We obtained 16S rDNA of 1246 bp and rp gene of 1212 bp, and sequence analysis showed a high identity with members of the 16SrI-C group phytoplasmas, all above 99.0%, and had the highest identity with Wheat Blue Dwarf phytoplasmas, 99.8% (16S rDNA) and 99.6% (rp) respectively, RFLP pattern are also the same as 16S rI-C phytoplsama.

Conclusion: Willow yellow phytoplasma is a member of 16S rI-C.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Ribosomal / genetics
  • Phytoplasma / classification*
  • Phytoplasma / genetics*
  • Plant Diseases / microbiology*
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length / genetics
  • Salix / microbiology*

Substances

  • DNA, Ribosomal