Isolation and mapping of bacterial artificial chromosome (BAC) clone containing telomere-associated sequence

X Gong; S Chen; J Gong; F Liu

doi:10.1007/BF02882903

Isolation and mapping of bacterial artificial chromosome (BAC) clone containing telomere-associated sequence

Sci China C Life Sci. 1998 Dec;41(6):617-22. doi: 10.1007/BF02882903.

Authors

X Gong¹, S Chen, J Gong, F Liu

Affiliation

¹ Institute of Genetics, Chinese Academy of Sciences, Beijing, China.

PMID: 18726217
DOI: 10.1007/BF02882903

Abstract

Using single primer (TTTAGGG)(3) or (CCCTAAA)(3)CCC corresponding to rice telomeric repeat sequences, two fragments were amplified from rice genomic DNA and named Tas1 and Tas2. These two fragments did not show polymorphism between the mapping parents. Tasl was found to hybridize to rice telomeric regions by in situ hybridization (FISH). Then a rice bacterial artificial chromosome (BAC) library was constructed and Tasl was used to screen it. One positive clone was identified. A single copy sequence of this BAC clone was mapped at the end of the 6th chromosome of rice.