Application of a single-drop microextraction for the analysis of organophosphorus pesticides in juice

J Chromatogr A. 2006 May 12;1114(2):269-73. doi: 10.1016/j.chroma.2006.03.011. Epub 2006 Mar 29.

Abstract

In this study, a new method for the determination of organophosphorus pesticides (OPPs) (ethoprophos, diazinon, parathion methyl, fenitrothion, malathion, isocarbophos and quinaphos) in orange juice was developed. Single-drop microextraction (SDME) parameters, such as organic solvent, drop volume, agitation rate, extraction time, and salt concentration were optimized through analysis of OPPs in fortified water. The orange juice was simply centrifuged and diluted with water, extracted by SDME and analyzed by gas chromatography (GC) equipped with a flame photometric detection (FPD). Fortification tests were conducted for concentrations between 10 and 500 microg/L; mean relative recoveries for each pesticide were all above 76.2% and below 108.0%. Limits of detection of the method for orange juice were below 5 microg/L for all target pesticides. The repeatability of the proposed method, expressed as relative standard deviation varied between 4.6 and 14.1% (n=5). The proposed method is acceptable in the analysis of OPPs pesticides in juice matrices.

Publication types

  • Validation Study

MeSH terms

  • Chromatography, Gas / methods
  • Citrus / chemistry*
  • Food Contamination / analysis*
  • Microchemistry / methods
  • Organophosphorus Compounds / analysis*
  • Pesticide Residues / analysis*
  • Photometry / methods
  • Reproducibility of Results

Substances

  • Organophosphorus Compounds
  • Pesticide Residues