Abstract
Purple acid phosphatase (PAP) was purified from sweet potato dry powder, which is used as a food additive. Spectrometric and enzymatic analyses, and analysis of the amino-terminal sequence indicated that the purified purple acid phosphatase was PAP1. High activity in neutral and acidic conditions, broad substrate specificity, and good thermal stability of PAP1 suggest the possibility of practical applications of PAP1.
MeSH terms
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Acid Phosphatase / chemistry
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Acid Phosphatase / isolation & purification*
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Acid Phosphatase / metabolism*
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Electrophoresis, Polyacrylamide Gel
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Enzyme Stability
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Glycoproteins / chemistry
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Glycoproteins / isolation & purification*
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Glycoproteins / metabolism*
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Ipomoea batatas / enzymology*
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Pancreatitis-Associated Proteins
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Substrate Specificity
Substances
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Glycoproteins
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Pancreatitis-Associated Proteins
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REG3A protein, human
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purple acid phosphatase
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Acid Phosphatase