An indirect enzyme-linked immunosorbent assay (ELISA) has been developed for evaluation of chapati-making quality of wheat varieties. Polyclonal antibodies against gliadin, low molecular weight glutenin (LMG), and high molecular weight glutenin (HMG) were developed and utilized in the ELISA. Twenty-eight Indian wheat varieties were utilized in the ELISA. Out of these antibodies, an antigliadin antibody response was negatively correlated with farinograph water absorption (r = -0.89 at P < 0.01), chapati dough water absorption (r = -0.91 at P < 0.01), overall chapati sensory score (r = -0.95 at P < 0.01), chapati puffed height score (r = -0.95 at P < 0.01), and positively correlated with shear value of chapati (r = 0.76 at P < 0.01). Anti-LMG antibody response was not correlated with any of these parameters, whereas anti-HMG response positively correlated with chapati dough water absorption (r = 0.44 at P < 0.05), farinograph water absorption (r = 0.45 at P < 0.05), and overall chapati sensory score (r = 0.44 at P < 0.05), and negatively correlated with shear value (r = -0.38 at P < 0.05) and chapati puffed height (r = -0.44 at P < 0.05). The results indicate that wheat varieties with good chapati-making quality were having less antigliadin antibody response.