Objective: To explore the molecular mechanism of Shuanglong Pill (SLP) in intervening atherosclerosis, mainly the effect of SLP on the mRNA expression of the platelet-derived growth factor-A (PDGF-A) and the monocyte chemoattractant protein-1 (MCP-1).
Methods: Using the hyperlipoprotein serum (HLS) of cultured rabbits' aortic smooth muscle cell (SMC) to build the atherosclerotic cell model, and medicated serum acting on the cell model by serum pharmacologic method. The effect of SLP medicated serum on the level of PDGF-A, MCP-1 mRNA expression were observed and analysed by means of reverse transcriptase-polymerase chain reaction assay.
Results: It was found that HLS stimulated mRNA expression of the PDGF-A and the MCP-1, but the medicated serum of SLP lowered their expression level.
Conclusion: SLP could inhibit the function of cellular auto-secreting growth factor, it is one of the possible pharmacologic mechanism in intervening atherosclerosis.