The purpose of this study was to investigate the histology of two small masticatory muscles from females and males of more than 70 years of age. By using immuno- and enzyme histochemistry the muscles were characterized by their fiber types and myosin heavy chain pattern. The observations were compared with similar studies of the masseter and temporalis muscles. Previously the two small muscles have been described based solely upon their gross anatomy. One muscle originates from the anterior, deep surface of the temporal fascia and inserts in the temporal tendon: the temporo-mandibular muscle (TM). The other muscle originates from the upper part of the temporal surface of the frontal process of the zygomatic bone and the adjacent part of the frontal bone and inserts in the temporal tendon: the zygomaticomandibular muscle (ZM). In the masseter, TM, and ZM, most of the autopsy samples contained an abundant number of fibers containing neonatal myosin heavy chains while in the temporal muscle specimens, such fibers were sparse and scattered. Electrophoresis followed by immuno-staining of Western blots supported the histochemical findings. There was no obvious correspondence between fiber typing based upon ATPase activity and the neonatal myosin heavy chain content in the muscle fibers. Neither did the fibers show accordance in their content of adult slow and fast myosin heavy chains and in their content of neonatal myosin heavy chain.