Deletion mapping of 14q32 in human neuroblastoma defines an 1,100-kb region of common allelic loss

Med Pediatr Oncol. 2000 Dec;35(6):522-5. doi: 10.1002/1096-911x(20001201)35:6<522::aid-mpo4>3.0.co;2-6.

Abstract

Background: We analyzed loss of heterozygosity (LOH) in 54 primary neuroblastomas (NBs) using 12 microsatellite markers on 14q32, and found that 31% (17/54) NBs showed LOH.

Procedure: The smallest region of overlap (SRO) was identified between D14S62 and D14S987.

Results: There was no statistical correlation between LOH and any clinicopathologic features, including age, stage, amplification of MYCN, and ploidy. A sequence-ready bacterial artificial chromosome (BAC) contig was constructed, and the minimum tiling path of six BACs covered the SRO; the physical length of this region was no larger than 1,000 kb.

Conclusions: Our findings support the existence of a putative tumor-related gene on 14q32 for the tumorigenesis of NB.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Child
  • Child, Preschool
  • Chromosome Deletion*
  • Chromosome Mapping*
  • Chromosomes, Human, Pair 14 / genetics*
  • Genes, Tumor Suppressor / genetics*
  • Humans
  • Infant
  • Loss of Heterozygosity
  • Neuroblastoma / genetics*