PubMed

Molecular characterization of hybridoma subclones spontaneously switching at high frequencies in vitro.

Iglesias-Ussel MD, Zavadil J, Scharff MD.

J Immunol Methods. 2009 Oct 31;350(1-2):71-8. Epub 2009 Jul 17.PMID: 19619554 [PubMed - indexed for MEDLINE]Related articles

V-region mutation in vitro, in vivo, and in silico reveal the importance of the enzymatic properties of AID and the sequence environment.

MacCarthy T, Kalis SL, Roa S, Pham P, Goodman MF, Scharff MD, Bergman A.

Proc Natl Acad Sci U S A. 2009 May 26;106(21):8629-34. Epub 2009 May 14.PMID: 19443686 [PubMed - indexed for MEDLINE]Related articlesFree article

H3 trimethyl K9 and H3 acetyl K9 chromatin modifications are associated with class switch recombination.

Kuang FL, Luo Z, Scharff MD.

Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5288-93. Epub 2009 Mar 10.PMID: 19276123 [PubMed - indexed for MEDLINE]Related articlesFree article

SHMTool: a webserver for comparative analysis of somatic hypermutation datasets.

Maccarthy T, Roa S, Scharff MD, Bergman A.

DNA Repair (Amst). 2009 Jan 1;8(1):137-41. Epub 2008 Nov 8.PMID: 18952008 [PubMed - indexed for MEDLINE]Related articles

Ubiquitylated PCNA plays a role in somatic hypermutation and class-switch recombination and is required for meiotic progression.

Roa S, Avdievich E, Peled JU, Maccarthy T, Werling U, Kuang FL, Kan R, Zhao C, Bergman A, Cohen PE, Edelmann W, Scharff MD.

Proc Natl Acad Sci U S A. 2008 Oct 21;105(42):16248-53. Epub 2008 Oct 14.PMID: 18854411 [PubMed - indexed for MEDLINE]Related articlesFree article

Does antisense make sense of AID targeting?

Roa S, Kuang FL, Scharff MD.

Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3661-2. Epub 2008 Mar 5. No abstract available. PMID: 18322011 [PubMed - indexed for MEDLINE]Related articlesFree article

The biochemistry of somatic hypermutation.

Peled JU, Kuang FL, Iglesias-Ussel MD, Roa S, Kalis SL, Goodman MF, Scharff MD.

Annu Rev Immunol. 2008;26:481-511. Review.PMID: 18304001 [PubMed - indexed for MEDLINE]Related articles

Human recombinant Fab fragments with sub-nanomolar affinities for acetylated histones.

Batova I, Kowal C, May R, Scharff MD, Diamond B.

J Immunol Methods. 2008 Jan 1;329(1-2):1-10. Epub 2007 Oct 11.PMID: 17976637 [PubMed - indexed for MEDLINE]Related articles

AID-initiated purposeful mutations in immunoglobulin genes.

Goodman MF, Scharff MD, Romesberg FE.

Adv Immunol. 2007;94:127-55. Review.PMID: 17560274 [PubMed - indexed for MEDLINE]Related articles

Targeting AID to the Ig genes.

Li Z, Luo Z, Ronai D, Kuang FL, Peled JU, Iglesias-Ussel MD, Scharff MD.

Adv Exp Med Biol. 2007;596:93-109. Review. No abstract available. PMID: 17338179 [PubMed - indexed for MEDLINE]Related articles

Detection of chromatin-associated single-stranded DNA in regions targeted for somatic hypermutation.

Ronai D, Iglesias-Ussel MD, Fan M, Li Z, Martin A, Scharff MD.

J Exp Med. 2007 Jan 22;204(1):181-90. Epub 2007 Jan 16.PMID: 17227912 [PubMed - indexed for MEDLINE]Related articlesFree article

Forced expression of AID facilitates the isolation of class switch variants from hybridoma cells.

Iglesias-Ussel MD, Fan M, Li Z, Martin A, Scharff MD.

J Immunol Methods. 2006 Oct 20;316(1-2):59-66. Epub 2006 Sep 5.PMID: 16997317 [PubMed - indexed for MEDLINE]Related articlesFree article

The mismatch repair protein Msh6 influences the in vivo AID targeting to the Ig locus.

Li Z, Zhao C, Iglesias-Ussel MD, Polonskaya Z, Zhuang M, Yang G, Luo Z, Edelmann W, Scharff MD.

Immunity. 2006 Apr;24(4):393-403.PMID: 16618598 [PubMed - indexed for MEDLINE]Related articles

A role for Mlh3 in somatic hypermutation.

Li Z, Peled JU, Zhao C, Svetlanov A, Ronai D, Cohen PE, Scharff MD.

DNA Repair (Amst). 2006 Jun 10;5(6):675-82. Epub 2006 Mar 27.PMID: 16564751 [PubMed - indexed for MEDLINE]Related articles

Tumor necrosis factor-alpha and CD40L modulate cell surface morphology and induce aggregation in Ramos Burkitt's lymphoma cells.

Laskov R, Berger N, Scharff MD, Horwitz MS.

Leuk Lymphoma. 2006 Mar;47(3):507-19.PMID: 16523591 [PubMed - indexed for MEDLINE]Related articles

Complex regulation of somatic hypermutation by cis-acting sequences in the endogenous IgH gene in hybridoma cells.

Ronai D, Iglesias-Ussel MD, Fan M, Shulman MJ, Scharff MD.

Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11829-34. Epub 2005 Aug 8.PMID: 16087866 [PubMed - indexed for MEDLINE]Related articlesFree article

Identifying protein-protein interactions in somatic hypermutation.

Goodman MF, Scharff MD.

J Exp Med. 2005 Feb 21;201(4):493-6. Epub 2005 Feb 14.PMID: 15710655 [PubMed - indexed for MEDLINE]Related articlesFree article

Variable-region-identical antibodies differing in isotype demonstrate differences in fine specificity and idiotype.

Torres M, May R, Scharff MD, Casadevall A.

J Immunol. 2005 Feb 15;174(4):2132-42.PMID: 15699144 [PubMed - indexed for MEDLINE]Related articlesFree article

Methylation protects cytidines from AID-mediated deamination.

Larijani M, Frieder D, Sonbuchner TM, Bransteitter R, Goodman MF, Bouhassira EE, Scharff MD, Martin A.

Mol Immunol. 2005 Mar;42(5):599-604.PMID: 15607819 [PubMed - indexed for MEDLINE]Related articles

Differential regulation of histone acetylation and generation of mutations in switch regions is associated with Ig class switching.

Li Z, Luo Z, Scharff MD.

Proc Natl Acad Sci U S A. 2004 Oct 26;101(43):15428-33. Epub 2004 Oct 14.PMID: 15486086 [PubMed - indexed for MEDLINE]Related articlesFree article

The role of activation-induced cytidine deaminase in antibody diversification, immunodeficiency, and B-cell malignancies.

Luo Z, Ronai D, Scharff MD.

J Allergy Clin Immunol. 2004 Oct;114(4):726-35; quiz 736. Review.PMID: 15480307 [PubMed - indexed for MEDLINE]Related articles

Examination of Msh6- and Msh3-deficient mice in class switching reveals overlapping and distinct roles of MutS homologues in antibody diversification.

Li Z, Scherer SJ, Ronai D, Iglesias-Ussel MD, Peled JU, Bardwell PD, Zhuang M, Lee K, Martin A, Edelmann W, Scharff MD.

J Exp Med. 2004 Jul 5;200(1):47-59.PMID: 15238604 [PubMed - indexed for MEDLINE]Related articlesFree article

The generation of antibody diversity through somatic hypermutation and class switch recombination.

Li Z, Woo CJ, Iglesias-Ussel MD, Ronai D, Scharff MD.

Genes Dev. 2004 Jan 1;18(1):1-11. Review. No abstract available. PMID: 14724175 [PubMed - indexed for MEDLINE]Related articlesFree article

Altered somatic hypermutation and reduced class-switch recombination in exonuclease 1-mutant mice.

Bardwell PD, Woo CJ, Wei K, Li Z, Martin A, Sack SZ, Parris T, Edelmann W, Scharff MD.

Nat Immunol. 2004 Feb;5(2):224-9. Epub 2004 Jan 11.PMID: 14716311 [PubMed - indexed for MEDLINE]Related articles

Msh2 ATPase activity is essential for somatic hypermutation at a-T basepairs and for efficient class switch recombination.

Martin A, Li Z, Lin DP, Bardwell PD, Iglesias-Ussel MD, Edelmann W, Scharff MD.

J Exp Med. 2003 Oct 20;198(8):1171-8.PMID: 14568978 [PubMed - indexed for MEDLINE]Related articlesFree article

Antibodies to keyhole limpet hemocyanin cross-react with an epitope on the polysaccharide capsule of Cryptococcus neoformans and other carbohydrates: implications for vaccine development.

May RJ, Beenhouwer DO, Scharff MD.

J Immunol. 2003 Nov 1;171(9):4905-12.PMID: 14568972 [PubMed - indexed for MEDLINE]Related articlesFree article

Induction of somatic hypermutation is associated with modifications in immunoglobulin variable region chromatin.

Woo CJ, Martin A, Scharff MD.

Immunity. 2003 Oct;19(4):479-89.PMID: 14563313 [PubMed - indexed for MEDLINE]Related articles

Mutations in AID and UNG extend the function of AID.

Li Z, Woo CJ, Scharff MD.

Nat Immunol. 2003 Oct;4(10):945-6. No abstract available. PMID: 14515126 [PubMed - indexed for MEDLINE]Related articles

Activation-induced cytidine deaminase deaminates deoxycytidine on single-stranded DNA but requires the action of RNase.

Bransteitter R, Pham P, Scharff MD, Goodman MF.

Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):4102-7. Epub 2003 Mar 21.PMID: 12651944 [PubMed - indexed for MEDLINE]Related articlesFree article

Cutting edge: the G-U mismatch glycosylase methyl-CpG binding domain 4 is dispensable for somatic hypermutation and class switch recombination.

Bardwell PD, Martin A, Wong E, Li Z, Edelmann W, Scharff MD.

J Immunol. 2003 Feb 15;170(4):1620-4.PMID: 12574322 [PubMed - indexed for MEDLINE]Related articlesFree article

High affinity mimotope of the polysaccharide capsule of Cryptococcus neoformans identified from an evolutionary phage peptide library.

Beenhouwer DO, May RJ, Valadon P, Scharff MD.

J Immunol. 2002 Dec 15;169(12):6992-9.PMID: 12471134 [PubMed - indexed for MEDLINE]Related articlesFree article

Somatic hypermutation of the AID transgene in B and non-B cells.

Martin A, Scharff MD.

Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12304-8. Epub 2002 Aug 29.PMID: 12202747 [PubMed - indexed for MEDLINE]Related articlesFree article

AID and mismatch repair in antibody diversification.

Martin A, Scharff MD.

Nat Rev Immunol. 2002 Aug;2(8):605-14. Review. No abstract available. PMID: 12154379 [PubMed - indexed for MEDLINE]Related articles

Mutation detection of immunoglobulin V-regions by DHPLC.

Bardwell PD, Martin A, Scharff MD.

J Immunol Methods. 2002 Aug 1;266(1-2):165-73.PMID: 12133633 [PubMed - indexed for MEDLINE]Related articles

Immunoglobulin G monoclonal antibodies to Cryptococcus neoformans protect mice deficient in complement component C3.

Shapiro S, Beenhouwer DO, Feldmesser M, Taborda C, Carroll MC, Casadevall A, Scharff MD.

Infect Immun. 2002 May;70(5):2598-604.PMID: 11953401 [PubMed - indexed for MEDLINE]Related articlesFree article

Activation-induced cytidine deaminase turns on somatic hypermutation in hybridomas.

Martin A, Bardwell PD, Woo CJ, Fan M, Shulman MJ, Scharff MD.

Nature. 2002 Feb 14;415(6873):802-6. Epub 2002 Jan 30.PMID: 11823785 [PubMed - indexed for MEDLINE]Related articles

Testing the reverse transcriptase model of somatic mutation.

Sack SZ, Bardwell PD, Scharff MD.

Mol Immunol. 2001 Aug;38(4):303-11.PMID: 11566323 [PubMed - indexed for MEDLINE]Related articles

Both Th1 and Th2 cytokines affect the ability of monoclonal antibodies to protect mice against Cryptococcus neoformans.

Beenhouwer DO, Shapiro S, Feldmesser M, Casadevall A, Scharff MD.

Infect Immun. 2001 Oct;69(10):6445-55.PMID: 11553589 [PubMed - indexed for MEDLINE]Related articlesFree article

Immunology. Antibody alterations.

Martin A, Scharff MD.

Nature. 2001 Aug 30;412(6850):870-1. No abstract available. PMID: 11528462 [PubMed - indexed for MEDLINE]Related articles

Clonal instability of V region hypermutation in the Ramos Burkitt's lymphoma cell line.

Zhang W, Bardwell PD, Woo CJ, Poltoratsky V, Scharff MD, Martin A.

Int Immunol. 2001 Sep;13(9):1175-84.PMID: 11526098 [PubMed - indexed for MEDLINE]Related articlesFree article

Expression of error-prone polymerases in BL2 cells activated for Ig somatic hypermutation.

Poltoratsky V, Woo CJ, Tippin B, Martin A, Goodman MF, Scharff MD.

Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):7976-81. Epub 2001 Jun 26.PMID: 11427727 [PubMed - indexed for MEDLINE]Related articlesFree article

Influence of affinity and antigen density on antibody localization in a modifiable tumor targeting model.

Zuckier LS, Berkowitz EZ, Sattenberg RJ, Zhao QH, Deng HF, Scharff MD.

Cancer Res. 2000 Dec 15;60(24):7008-13.PMID: 11156404 [PubMed - indexed for MEDLINE]Related articlesFree article

Error-prone candidates vie for somatic mutation.

Poltoratsky V, Goodman MF, Scharff MD.

J Exp Med. 2000 Nov 20;192(10):F27-30. No abstract available. PMID: 11085756 [PubMed - indexed for MEDLINE]Related articlesFree article

Somatic hypermutation in MutS homologue (MSH)3-, MSH6-, and MSH3/MSH6-deficient mice reveals a role for the MSH2-MSH6 heterodimer in modulating the base substitution pattern.

Wiesendanger M, Kneitz B, Edelmann W, Scharff MD.

J Exp Med. 2000 Feb 7;191(3):579-84.PMID: 10662804 [PubMed - indexed for MEDLINE]Related articlesFree article

Molecular and idiotypic analyses of the antibody response to Cryptococcus neoformans glucuronoxylomannan-protein conjugate vaccine in autoimmune and nonautoimmune mice.

Nussbaum G, Anandasabapathy S, Mukherjee J, Fan M, Casadevall A, Scharff MD.

Infect Immun. 1999 Sep;67(9):4469-76.PMID: 10456888 [PubMed - indexed for MEDLINE]Related articlesFree article

Chimeric human-mouse IgG antibodies with shuffled constant region exons demonstrate that multiple domains contribute to in vivo half-life.

Zuckier LS, Chang CJ, Scharff MD, Morrison SL.

Cancer Res. 1998 Sep 1;58(17):3905-8.PMID: 9731501 [PubMed - indexed for MEDLINE]Related articlesFree article

Somatic hypermutation, transcription, and DNA mismatch repair.

Wiesendanger M, Scharff MD, Edelmann W.

Cell. 1998 Aug 21;94(4):415-8. Review. No abstract available. PMID: 9727483 [PubMed - indexed for MEDLINE]Related articles

The effects of E mu, 3'alpha (hs 1,2) and 3'kappa enhancers on mutation of an Ig-VDJ-Cgamma2a Ig heavy gene in cultured B cells.

Lin MM, Green NS, Zhang W, Scharff MD.

Int Immunol. 1998 Aug;10(8):1121-9.PMID: 9723698 [PubMed - indexed for MEDLINE]Related articlesFree article

Aspects of antigen mimicry revealed by immunization with a peptide mimetic of Cryptococcus neoformans polysaccharide.

Valadon P, Nussbaum G, Oh J, Scharff MD.

J Immunol. 1998 Aug 15;161(4):1829-36.PMID: 9712050 [PubMed - indexed for MEDLINE]Related articlesFree article

Somatic hypermutation of antibody genes: a hot spot warms up.

Green NS, Lin MM, Scharff MD.

Bioessays. 1998 Mar;20(3):227-34. Review.PMID: 9631650 [PubMed - indexed for MEDLINE]Related articles