PubMed

Diabetic neuropathy: Electrophysiological and morphological study of peripheral nerve degeneration and regeneration in transgenic mice that express IFNbeta in beta cells.

Serafín A, Molín J, Márquez M, Blasco E, Vidal E, Foradada L, Añor S, Rabanal RM, Fondevila D, Bosch F, Pumarola M.

Muscle Nerve. 2009 Nov 13. [Epub ahead of print]PMID: 19918773 [PubMed - as supplied by publisher]Related articles

HPV vaccination against cervical cancer in women above 25 years of age: key considerations and current perspectives.

Castellsagué X, Schneider A, Kaufmann AM, Bosch FX.

Gynecol Oncol. 2009 Dec;115(3 Suppl):S15-23. Epub 2009 Oct 12.PMID: 19819540 [PubMed - in process]Related articles

Optimal strategies for the eradication of asiatic citrus canker in heterogeneous host landscapes.

Parnell S, Gottwald TR, van den Bosch F, Gilligan CA.

Phytopathology. 2009 Dec;99(12):1370-6.PMID: 19900003 [PubMed - in process]Related articles

Salvage chemotherapy with alternating MINE-ESHAP regimen in relapsed or refractory Hodgkin's lymphoma followed by autologous stem-cell transplantation.

Fernández de Larrea C, Martínez C, Gaya A, López-Guillermo A, Rovira M, Fernández-Avilés F, Lozano M, Bosch F, Esteve J, Nomdedeu B, Montserrat E, Carreras E.

Ann Oncol. 2009 Nov 4. [Epub ahead of print]PMID: 19889622 [PubMed - as supplied by publisher]Related articles

Epigenetic silencing of interferon-kappa in human papillomavirus type 16-positive cells.

Rincon-Orozco B, Halec G, Rosenberger S, Muschik D, Nindl I, Bachmann A, Ritter TM, Dondog B, Ly R, Bosch FX, Zawatzky R, Rösl F.

Cancer Res. 2009 Nov 15;69(22):8718-25. Epub 2009 Nov 3.PMID: 19887612 [PubMed - in process]Related articles

Evaluation of quadrivalent HPV 6/11/16/18 vaccine efficacy against cervical and anogenital disease in subjects with serological evidence of prior vaccine type HPV infection.

Olsson SE, Kjaer SK, Sigurdsson K, Iversen OE, Hernandez-Avila M, Wheeler CM, Perez G, Brown DR, Koutsky LA, Tay EH, García P, Ault KA, Garland SM, Leodolter S, Tang GW, Ferris DG, Paavonen J, Lehtinen M, Steben M, Bosch FX, Dillner J, Joura EA, Majewski S, Muñoz N, Myers ER, Villa LL, Taddeo FJ, Roberts C, Tadesse A, Bryan J, Maansson R, Vuocolo S, Hesley TM, Saah A, Barr E, Haupt RM.

Hum Vaccin. 2009 Oct 14;5(10). [Epub ahead of print]PMID: 19855170 [PubMed - as supplied by publisher]Related articles

Human papillomavirus types in invasive cervical cancer specimens from Turkey.

Usubütün A, Alemany L, Küçükali T, Ayhan A, Yüce K, de Sanjosé S, Font R, Lloveras B, Klaustermeier J, Quint W, Muñoz N, Bosch FX.

Int J Gynecol Pathol. 2009 Nov;28(6):541-8.PMID: 19851201 [PubMed - in process]Related articles

Over-expression of kinase negative protein kinase C{delta} in pancreatic {beta}-cells protects mice from diet-induced glucose intolerance and {beta}-cell dysfunction.

Hennige AM, Ranta F, Heinzelmann I, Düfer M, Michael D, Braumüller H, Lutz SZ, Lammers R, Drews G, Bosch F, Häring HU, Ullrich S.

Diabetes. 2009 Oct 13. [Epub ahead of print]PMID: 19826167 [PubMed - as supplied by publisher]Related articles

Effectiveness of adalimumab in treating patients with active psoriatic arthritis (PsA) and predictors of good clinical responses for arthritis, skin, and nail lesions.

van den Bosch F, Manger B, Goupille P, McHugh N, Rødevand E, Holck P, van Vollenhoven RF, Leirisalo-Repo M, Fitzgerald O, Kron M, Frank M, Kary S, Kupper H.

Ann Rheum Dis. 2009 Oct 7. [Epub ahead of print]PMID: 19815494 [PubMed - as supplied by publisher]Related articlesFree article

A pooled analysis of continued prophylactic efficacy of quadrivalent human papillomavirus (Types 6/11/16/18) vaccine against high-grade cervical and external genital lesions.

Kjaer SK, Sigurdsson K, Iversen OE, Hernandez-Avila M, Wheeler CM, Perez G, Brown DR, Koutsky LA, Tay EH, García P, Ault KA, Garland SM, Leodolter S, Olsson SE, Tang GW, Ferris DG, Paavonen J, Lehtinen M, Steben M, Bosch FX, Dillner J, Joura EA, Majewski S, Muñoz N, Myers ER, Villa LL, Taddeo FJ, Roberts C, Tadesse A, Bryan J, Maansson R, Lu S, Vuocolo S, Hesley TM, Saah A, Barr E, Haupt RM.

Cancer Prev Res (Phila Pa). 2009 Oct;2(10):868-78. Epub 2009 Sep 29.PMID: 19789295 [PubMed - in process]Related articles

Intranasal administration of recombinant Human Cartilage glycoprotein-39 as a treatment for rheumatoid arthritis: A Phase II, multicenter, double-blind, randomized, placebo-controlled, parallel group dose-finding trial.

Landewé RB, Houbiers JG, Van den Bosch FE, In 't Hout J, Verschueren PC, Meijerink JH, van den Hoogen FH, Masek BA, Bruyn GA, Wouters JM, Voskuyl AE, van Laar JM, Bijlsma JJ, van der Heijde DM, Breedveld FC, van de Putte LB, Miltenburg AM, De Keyser F.

Ann Rheum Dis. 2009 Sep 23. [Epub ahead of print]PMID: 19778914 [PubMed - as supplied by publisher]Related articles

Renal infarction after cocaine abuse: a case report and review.

Hoefsloot W, de Vries RA, Bruijnen R, Bosch FH.

Clin Nephrol. 2009 Sep;72(3):234-6.PMID: 19761732 [PubMed - in process]Related articles

Gene and cellular therapy in Spain: moving forward.

Bosch F.

Hum Gene Ther. 2009 Sep;20(9):919. No abstract available. PMID: 19739942 [PubMed - in process]Related articles

Metabolic acidosis improves airway conductance in patients with asthma.

Brijker F, van den Elshout FJ, Bosch FH, Heijdra YF, Folgering HT.

J Asthma. 2009 Sep;46(7):656-8.PMID: 19728200 [PubMed - indexed for MEDLINE]Related articles

Human papillomavirus prevalence and type distribution in penile carcinoma.

Miralles-Guri C, Bruni L, Cubilla AL, Castellsagué X, Bosch FX, de Sanjosé S.

J Clin Pathol. 2009 Oct;62(10):870-8. Epub 2009 Aug 25.PMID: 19706632 [PubMed - in process]Related articles

Rituximab, fludarabine, cyclophosphamide, and mitoxantrone: a new, highly active chemoimmunotherapy regimen for chronic lymphocytic leukemia.

Bosch F, Abrisqueta P, Villamor N, Terol MJ, González-Barca E, Ferra C, González Diaz M, Abella E, Delgado J, Carbonell F, García Marco JA, Escoda L, Ferrer S, Monzó E, González Y, Estany C, Jarque I, Salamero O, Muntañola A, Montserrat E.

J Clin Oncol. 2009 Sep 20;27(27):4578-84. Epub 2009 Aug 24.PMID: 19704063 [PubMed - indexed for MEDLINE]Related articles

Gene expression profiling in chronic lymphocytic leukaemia.

Codony C, Crespo M, Abrisqueta P, Montserrat E, Bosch F.

Best Pract Res Clin Haematol. 2009 Jun;22(2):211-22. Review.PMID: 19698929 [PubMed - indexed for MEDLINE]Related articles

Phosphofructo-1-kinase deficiency leads to a severe cardiac and hematological disorder in addition to skeletal muscle glycogenosis.

García M, Pujol A, Ruzo A, Riu E, Ruberte J, Arbós A, Serafín A, Albella B, Felíu JE, Bosch F.

PLoS Genet. 2009 Aug;5(8):e1000615. Epub 2009 Aug 21.PMID: 19696889 [PubMed - in process]Related articlesFree article

Constitutive expression of suppressor of cytokine signalling-3 in skeletal muscle leads to reduced mobility and overweight in mice.

Lebrun P, Cognard E, Bellon-Paul R, Gontard P, Filloux C, Jehl-Pietri C, Grimaldi P, Samson M, Pénicaud L, Ruberte J, Ferre T, Pujol A, Bosch F, Van Obberghen E.

Diabetologia. 2009 Oct;52(10):2201-12. Epub 2009 Aug 12.PMID: 19672574 [PubMed - in process]Related articles

Improvement in hemoglobin levels in patients with ankylosing spondylitis treated with infliximab.

Braun J, van der Heijde D, Doyle MK, Han C, Deodhar A, Inman R, de Vlam K, Burmester GR, Van den Bosch F, Xu S, Visvanathan S, Rahman MU.

Arthritis Rheum. 2009 Aug 15;61(8):1032-6.PMID: 19644896 [PubMed - indexed for MEDLINE]Related articles

Respiratory muscle strength and muscle endurance are not affected by acute metabolic acidemia.

Nizet TA, Heijdra YF, van den Elshout FJ, van de Ven MJ, Bosch FH, Mulder PH, Folgering HT.

Clin Physiol Funct Imaging. 2009 Oct;29(6):392-9. Epub 2009 Jul 10.PMID: 19624691 [PubMed - in process]Related articles

Resident autofluorescent perivascular macrophages have a scavenger function and may contribute to the maintenance of the blood-retinal barrier.

Mendes-Jorge L, Ramos D, Luppo M, Llombart C, Alexandre-Pires G, Nacher V, Melgarejo V, Correia M, Navarro M, Carretero A, Tafuro S, Rodriguez-Baeza A, Esperança-Pina JA, Bosch F, Ruberte J.

Invest Ophthalmol Vis Sci. 2009 Jul 15. [Epub ahead of print]PMID: 19608545 [PubMed - as supplied by publisher]Related articles

Efficacy of human papillomavirus (HPV)-16/18 AS04-adjuvanted vaccine against cervical infection and precancer caused by oncogenic HPV types (PATRICIA): final analysis of a double-blind, randomised study in young women.

Paavonen J, Naud P, Salmerón J, Wheeler CM, Chow SN, Apter D, Kitchener H, Castellsague X, Teixeira JC, Skinner SR, Hedrick J, Jaisamrarn U, Limson G, Garland S, Szarewski A, Romanowski B, Aoki FY, Schwarz TF, Poppe WA, Bosch FX, Jenkins D, Hardt K, Zahaf T, Descamps D, Struyf F, Lehtinen M, Dubin G; HPV PATRICIA Study Group, Greenacre M.

Lancet. 2009 Jul 25;374(9686):301-14. Epub 2009 Jul 6.PMID: 19586656 [PubMed - indexed for MEDLINE]Related articles

Improving survival in patients with chronic lymphocytic leukemia (1980-2008): the Hospital Clinic of Barcelona experience.

Abrisqueta P, Pereira A, Rozman C, Aymerich M, Giné E, Moreno C, Muntañola A, Rozman M, Villamor N, Hodgson K, Campo E, Bosch F, Montserrat E.

Blood. 2009 Sep 3;114(10):2044-50. Epub 2009 Jun 24.PMID: 19553638 [PubMed - indexed for MEDLINE]Related articles

The effect of transmission route on plant virus epidemic development and disease control.

Jeger MJ, Madden LV, van den Bosch F.

J Theor Biol. 2009 May 21;258(2):198-207. Epub 2009 Jan 21.PMID: 19490879 [PubMed - indexed for MEDLINE]Related articles

New opportunities for cancer prevention.

Bosch FX.

Vaccine. 2009 May 29;27 Suppl 1:A2-3. No abstract available. PMID: 19480956 [PubMed - indexed for MEDLINE]Related articles

Increased intraocular insulin-like growth factor-I triggers blood-retinal barrier breakdown.

Haurigot V, Villacampa P, Ribera A, Llombart C, Bosch A, Nacher V, Ramos D, Ayuso E, Segovia JC, Bueren JA, Ruberte J, Bosch F.

J Biol Chem. 2009 Aug 21;284(34):22961-9. Epub 2009 May 27.PMID: 19473988 [PubMed - indexed for MEDLINE]Related articles

Human Papillomavirus (HPV) infection in pregnant women and mother-to-child transmission of genital HPV genotypes: a prospective study in Spain.

Castellsagué X, Drudis T, Cañadas MP, Goncé A, Ros R, Pérez JM, Quintana MJ, Muñoz J, Albero G, de Sanjosé S, Bosch FX.

BMC Infect Dis. 2009 May 27;9:74.PMID: 19473489 [PubMed - indexed for MEDLINE]Related articlesFree article

Broad-spectrum human papillomavirus vaccines: new horizons but one step at a time.

Bosch FX.

J Natl Cancer Inst. 2009 Jun 3;101(11):771-3. Epub 2009 May 26. No abstract available. PMID: 19470950 [PubMed - indexed for MEDLINE]Related articles

The impact of a quadrivalent human papillomavirus (types 6, 11, 16, 18) virus-like particle vaccine in European women aged 16 to 24.

Majewski S, Bosch FX, Dillner J, Iversen OE, Kjaer SK, Muñoz N, Olsson SE, Paavonen J, Sigurdsson K, Bryan J, Esser MT, Giacoletti K, James M, Taddeo F, Vuocolo S, Barr E.

J Eur Acad Dermatol Venereol. 2009 Oct;23(10):1147-55. Epub 2009 Apr 23.PMID: 19453788 [PubMed - in process]Related articles

Spinal radiographic changes in ankylosing spondylitis: association with clinical characteristics and functional outcome.

Boonen A, vander Cruyssen B, de Vlam K, Steinfeld S, Ribbens C, Lenaerts J, Van den Bosch F, Mielants H, Dewulf L, Vastesaeger N.

J Rheumatol. 2009 Jun;36(6):1249-55. Epub 2009 May 15.PMID: 19447933 [PubMed - indexed for MEDLINE]Related articles

High prevalence of human papillomavirus infection in the female population of Guatemala.

Vallès X, Murga GB, Hernández G, Sabidó M, Chuy A, Lloveras B, Alameda F, de San José S, Bosch FX, Pedroza I, Castellsagué X, Casabona J.

Int J Cancer. 2009 Sep 1;125(5):1161-7.PMID: 19415744 [PubMed - indexed for MEDLINE]Related articles

Enforced expression of protein kinase C in skeletal muscle causes physical inactivity, fatty liver and insulin resistance in the brain.

Hennige AM, Heni M, Machann J, Staiger H, Sartorius T, Hoene M, Lehmann R, Weigert C, Peter A, Bornemann A, Kroeber S, Pujol A, Franckhauser S, Bosch F, Schick F, Lammers R, Håring HU.

J Cell Mol Med. 2008 Dec 24. [Epub ahead of print]PMID: 19413892 [PubMed - as supplied by publisher]Related articles

Intracellular signaling pathways control mitochondrial events associated with the development of ischemia/ reperfusion-associated damage.

Sucher R, Gehwolf P, Kaier T, Hermann M, Maglione M, Oberhuber R, Ratschiller T, Kuznetsov AV, Bösch F, Kozlov AV, Ashraf MI, Schneeberger S, Brandacher G, Ollinger R, Margreiter R, Troppmair J.

Transpl Int. 2009 Sep;22(9):922-30. Epub 2009 Apr 28.PMID: 19413579 [PubMed - indexed for MEDLINE]Related articles

Pre-emptive anti-hyperalgesic effect of electroacupuncture in carrageenan-induced inflammation: role of nitric oxide.

Garrido-Suárez BB, Garrido G, Márquez L, Martínez I, Hernández I, Merino N, Luque Y, Delgado R, Bosch F.

Brain Res Bull. 2009 Aug 14;79(6):339-44. Epub 2009 May 3.PMID: 19410637 [PubMed - indexed for MEDLINE]Related articles

Schottky mass measurement of the 208Hg isotope: implication for the proton-neutron interaction strength around doubly magic 208Pb.

Chen L, Litvinov YA, Plass WR, Beckert K, Beller P, Bosch F, Boutin D, Caceres L, Cakirli RB, Carroll JJ, Casten RF, Chakrawarthy RS, Cullen DM, Cullen IJ, Franzke B, Geissel H, Gerl J, Górska M, Jones GA, Kishada A, Knöbel R, Kozhuharov C, Litvinov SA, Liu Z, Mandal S, Montes F, Münzenberg G, Nolden F, Ohtsubo T, Patyk Z, Podolyák Z, Propri R, Rigby S, Saito N, Saito T, Scheidenberger C, Shindo M, Steck M, Ugorowski P, Walker PM, Williams S, Weick H, Winkler M, Wollersheim HJ, Yamaguchi T.

Phys Rev Lett. 2009 Mar 27;102(12):122503. Epub 2009 Mar 27. Erratum in: Phys Rev Lett. 2009 Apr 3;102(13):139904. PMID: 19392270 [PubMed]Related articles

[Obtaining medical ethical approval for a multicentre, randomised study: prospective evaluation of a ponderous process]

Bosch FH, Klazinga NS, Schaffmeister BE.

Ned Tijdschr Geneeskd. 2009 Jan 24;153(4):154. Dutch. No abstract available. PMID: 19348140 [PubMed - indexed for MEDLINE]Related articles

[Obtaining medical ethical approval for a multicentre, randomised study: prospective evaluation of a ponderous process]

Bosch FH, Klazinga NS, Schaffmeister BE.

Ned Tijdschr Geneeskd. 2009 Feb 14;153(7):310; author reply 310. Dutch. No abstract available. PMID: 19291950 [PubMed - indexed for MEDLINE]Related articles

Early age at first sexual intercourse and early pregnancy are risk factors for cervical cancer in developing countries.

Louie KS, de Sanjose S, Diaz M, Castellsagué X, Herrero R, Meijer CJ, Shah K, Franceschi S, Muñoz N, Bosch FX; International Agency for Research on Cancer Multicenter Cervical Cancer Study Group.

Br J Cancer. 2009 Apr 7;100(7):1191-7. Epub 2009 Mar 10.PMID: 19277042 [PubMed - indexed for MEDLINE]Related articles

The impact of quadrivalent human papillomavirus (HPV; types 6, 11, 16, and 18) L1 virus-like particle vaccine on infection and disease due to oncogenic nonvaccine HPV types in generally HPV-naive women aged 16-26 years.

Brown DR, Kjaer SK, Sigurdsson K, Iversen OE, Hernandez-Avila M, Wheeler CM, Perez G, Koutsky LA, Tay EH, Garcia P, Ault KA, Garland SM, Leodolter S, Olsson SE, Tang GW, Ferris DG, Paavonen J, Steben M, Bosch FX, Dillner J, Joura EA, Kurman RJ, Majewski S, Muñoz N, Myers ER, Villa LL, Taddeo FJ, Roberts C, Tadesse A, Bryan J, Lupinacci LC, Giacoletti KE, Sings HL, James M, Hesley TM, Barr E.

J Infect Dis. 2009 Apr 1;199(7):926-35.PMID: 19236279 [PubMed - indexed for MEDLINE]Related articles

The impact of quadrivalent human papillomavirus (HPV; types 6, 11, 16, and 18) L1 virus-like particle vaccine on infection and disease due to oncogenic nonvaccine HPV types in sexually active women aged 16-26 years.

Wheeler CM, Kjaer SK, Sigurdsson K, Iversen OE, Hernandez-Avila M, Perez G, Brown DR, Koutsky LA, Tay EH, García P, Ault KA, Garland SM, Leodolter S, Olsson SE, Tang GW, Ferris DG, Paavonen J, Steben M, Bosch FX, Dillner J, Joura EA, Kurman RJ, Majewski S, Muñoz N, Myers ER, Villa LL, Taddeo FJ, Roberts C, Tadesse A, Bryan J, Lupinacci LC, Giacoletti KE, James M, Vuocolo S, Hesley TM, Barr E.

J Infect Dis. 2009 Apr 1;199(7):936-44.PMID: 19236277 [PubMed - indexed for MEDLINE]Related articles

The dynamic DNA methylomes of double-stranded DNA viruses associated with human cancer.

Fernandez AF, Rosales C, Lopez-Nieva P, Graña O, Ballestar E, Ropero S, Espada J, Melo SA, Lujambio A, Fraga MF, Pino I, Javierre B, Carmona FJ, Acquadro F, Steenbergen RD, Snijders PJ, Meijer CJ, Pineau P, Dejean A, Lloveras B, Capella G, Quer J, Buti M, Esteban JI, Allende H, Rodriguez-Frias F, Castellsague X, Minarovits J, Ponce J, Capello D, Gaidano G, Cigudosa JC, Gomez-Lopez G, Pisano DG, Valencia A, Piris MA, Bosch FX, Cahir-McFarland E, Kieff E, Esteller M.

Genome Res. 2009 Mar;19(3):438-51. Epub 2009 Feb 10.PMID: 19208682 [PubMed - indexed for MEDLINE]Related articlesFree article

[Serious and sometimes fatal consequences of high-altitude pulmonary oedema]

Berendsen RR, Willems JH, Bosch FH, Hulsebosch R, Kayser B.

Ned Tijdschr Geneeskd. 2008 Dec 20;152(51-52):2758-62. Dutch. PMID: 19177913 [PubMed - indexed for MEDLINE]Related articles

Male circumcision, human papillomavirus and cervical cancer: from evidence to intervention.

Bosch FX, Albero G, Castellsagué X.

J Fam Plann Reprod Health Care. 2009 Jan;35(1):5-7. No abstract available. PMID: 19126309 [PubMed - in process]Related articles

Decontamination of the digestive tract and oropharynx in ICU patients.

de Smet AM, Kluytmans JA, Cooper BS, Mascini EM, Benus RF, van der Werf TS, van der Hoeven JG, Pickkers P, Bogaers-Hofman D, van der Meer NJ, Bernards AT, Kuijper EJ, Joore JC, Leverstein-van Hall MA, Bindels AJ, Jansz AR, Wesselink RM, de Jongh BM, Dennesen PJ, van Asselt GJ, te Velde LF, Frenay IH, Kaasjager K, Bosch FH, van Iterson M, Thijsen SF, Kluge GH, Pauw W, de Vries JW, Kaan JA, Arends JP, Aarts LP, Sturm PD, Harinck HI, Voss A, Uijtendaal EV, Blok HE, Thieme Groen ES, Pouw ME, Kalkman CJ, Bonten MJ.

N Engl J Med. 2009 Jan 1;360(1):20-31.PMID: 19118302 [PubMed - indexed for MEDLINE]Related articlesFree article

The presence of generalist plant pathogens might not explain the long-term coexistence of plant species.

van den Berg F, van den Bosch F.

J Theor Biol. 2009 Apr 7;257(3):446-53. Epub 2008 Dec 6.PMID: 19108783 [PubMed - in process]Related articles

Genetic variants in apoptosis and immunoregulation-related genes are associated with risk of chronic lymphocytic leukemia.

Enjuanes A, Benavente Y, Bosch F, Martín-Guerrero I, Colomer D, Pérez-Alvarez S, Reina O, Ardanaz MT, Jares P, García-Orad A, Pujana MA, Montserrat E, de Sanjosé S, Campo E.

Cancer Res. 2008 Dec 15;68(24):10178-86.PMID: 19074885 [PubMed - indexed for MEDLINE]Related articles

Human papillomavirus vaccines and vaccine implementation.

de Sanjosé S, Alemany L, Castellsagué X, Bosch FX.

Womens Health (Lond Engl). 2008 Nov;4(6):595-604. Review.PMID: 19072462 [PubMed - indexed for MEDLINE]Related articles

ASDAS, a highly discriminatory ASAS-endorsed disease activity score in patients with ankylosing spondylitis.

van der Heijde D, Lie E, Kvien TK, Sieper J, Van den Bosch F, Listing J, Braun J, Landewé R; Assessment of SpondyloArthritis international Society (ASAS).

Ann Rheum Dis. 2009 Dec;68(12):1811-8. Epub 2008 Dec 5.PMID: 19060001 [PubMed - in process]Related articles

Cost-effectiveness of vaccination against cervical cancer: a multi-regional analysis assessing the impact of vaccine characteristics and alternative vaccination scenarios.

Suárez E, Smith JS, Bosch FX, Nieminen P, Chen CJ, Torvinen S, Demarteau N, Standaert B.

Vaccine. 2008 Sep 15;26 Suppl 5:F29-45.PMID: 18992380 [PubMed - indexed for MEDLINE]Related articles