DPB2 DNA polymerase epsilon noncatalytic subunit [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: DPB2
Gene description: DNA polymerase epsilon noncatalytic subunit
Primary source: SGD:S000006379
Locus tag: YPR175W
See related: AllianceGenome:SGD:S000006379
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Contributes to DNA binding activity; DNA polymerase processivity factor activity; and DNA-directed DNA polymerase activity. Involved in DNA-dependent DNA replication maintenance of fidelity and error-prone translesion synthesis. Located in cytoplasm and nucleus. Part of epsilon DNA polymerase complex. Orthologous to human POLE2 (DNA polymerase epsilon 2, accessory subunit). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (888974..891043)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Impairment of the non-catalytic subunit Dpb2 of DNA Pol varepsilon results in increased involvement of Pol delta on the leading strand.
Title: Impairment of the non-catalytic subunit Dpb2 of DNA Pol ɛ results in increased involvement of Pol δ on the leading strand.
Dpb2, which participates in the early stages of DNA replication initiation, has an impact on the regulation of replication-related genes expression with possible implications for genomic stability. (Review)
Title: Diverse roles of Dpb2, the non-catalytic subunit of DNA polymerase ε.
dpb2-103 mutant cells progress through S phase faster than wild-type cells. Nrm1-dependent branch of the checkpoint is impaired in dpb2-103 cells.
Title: Mutations in the Non-Catalytic Subunit Dpb2 of DNA Polymerase Epsilon Affect the Nrm1 Branch of the DNA Replication Checkpoint.
The presence of the dpb2-100 mutant significantly decreases the interaction of Dpb2p with Psf1p and Psf3p.
Title: Fidelity consequences of the impaired interaction between DNA polymerase epsilon and the GINS complex.
Dpb2 integrates the leading-strand DNA polymerase into the eukaryotic replisome.
Title: Dpb2 integrates the leading-strand DNA polymerase into the eukaryotic replisome.
Authors propose that the essential function of Dpb2 is separate from the enzymatic activity of Pol epsilon in S. cerevisiae and that Dpb2 depends on a distinct domain at the very C-terminal end of the protein to physically interact with Pol2.
Title: The C-terminus of Dpb2 is required for interaction with Pol2 and for cell viability.
This DNA polymerase subunit other than those housing the DNA polymerase and 3' --> 5' exonuclease is essential in controlling the level of spontaneous mutagenesis and genetic stability in yeast cells.
Title: Dpb2p, a noncatalytic subunit of DNA polymerase epsilon, contributes to the fidelity of DNA replication in Saccharomyces cerevisiae.
mechanism for interaction of Pol epsilon with DNA that might explain how the structure of the enzyme contributes to its intrinsic processivity
Title: Structure of Saccharomyces cerevisiae DNA polymerase epsilon by cryo-electron microscopy.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA-templated DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA-templated DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA-templated DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA-templated DNA replication maintenance of fidelity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell cycle	IEA Inferred from Electronic Annotation more info
involved_in error-prone translesion synthesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in error-prone translesion synthesis	IDA Inferred from Direct Assay more info	PubMed
involved_in error-prone translesion synthesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of epsilon DNA polymerase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of epsilon DNA polymerase complex	IDA Inferred from Direct Assay more info	PubMed
part_of epsilon DNA polymerase complex	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA polymerase epsilon noncatalytic subunit

NP_015501.2

Second largest subunit of DNA polymerase II (DNA polymerase epsilon); required for maintenance of fidelity of chromosomal replication; essential motif in C-terminus is required for formation of the four-subunit Pol epsilon; expression peaks at the G1/S phase boundary; Cdc28p substrate

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

888974..891043

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184272.1 → NP_015501.2 TPA: DNA polymerase epsilon noncatalytic subunit [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015501.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W4H6, P24482, Q06622

UniProtKB/TrEMBL

A0A8H4BUB9

Conserved Domains (1) summary

pfam04042
Location:401 → 650

DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B