PubMed

Rapid single step subcloning procedure by combined action of type II and type IIs endonucleases with ligase.

Fromme T, Klingenspor M.

J Biol Eng. 2007 Nov 26;1:7.PMID: 18271995 [PubMed - in process]Related articlesFree article

A high-throughput and single-tube recombination of crude PCR products using a DNA polymerase inhibitor and type IIS restriction enzyme.

Kotera I, Nagai T.

J Biotechnol. 2008 Oct 10;137(1-4):1-7. Epub 2008 Jul 23.PMID: 18692529 [PubMed - indexed for MEDLINE]Related articles

Vectors with hidden cloning sites.

Welker E, Váradi A.

Biochem Biophys Res Commun. 2000 May 10;271(2):534-6.PMID: 10799330 [PubMed - indexed for MEDLINE]Related articles

A one pot, one step, precision cloning method with high throughput capability.

Engler C, Kandzia R, Marillonnet S.

PLoS One. 2008;3(11):e3647. Epub 2008 Nov 5.PMID: 18985154 [PubMed - indexed for MEDLINE]Related articlesFree article

Creation of a type IIS restriction endonuclease with a long recognition sequence.

Lippow SM, Aha PM, Parker MH, Blake WJ, Baynes BM, Lipovsek D.

Nucleic Acids Res. 2009 May;37(9):3061-73. Epub 2009 Mar 20.PMID: 19304757 [PubMed - indexed for MEDLINE]Related articlesFree article

Rapid one-step recombinational cloning.

Fu C, Wehr DR, Edwards J, Hauge B.

Nucleic Acids Res. 2008 May;36(9):e54. Epub 2008 Apr 19.PMID: 18424799 [PubMed - indexed for MEDLINE]Related articlesFree article

Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes.

Engler C, Gruetzner R, Kandzia R, Marillonnet S.

PLoS One. 2009;4(5):e5553. Epub 2009 May 14.PMID: 19436741 [PubMed - indexed for MEDLINE]Related articlesFree article

Direct subcloning of target region from BAC insert using restriction enzymes that produce non-identical cohesive ends.

Ohmi Y, Sato M, Ohtsuka M.

Nucleic Acids Symp Ser (Oxf). 2004;(48):141-2.PMID: 17150518 [PubMed - indexed for MEDLINE]Related articlesFree article

USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products.

Geu-Flores F, Nour-Eldin HH, Nielsen MT, Halkier BA.

Nucleic Acids Res. 2007;35(7):e55. Epub 2007 Mar 27.PMID: 17389646 [PubMed - indexed for MEDLINE]Related articlesFree article

Homologous recombination-mediated cloning and manipulation of genomic DNA regions using Gateway and recombineering systems.

Rozwadowski K, Yang W, Kagale S.

BMC Biotechnol. 2008 Nov 17;8:88.PMID: 19014699 [PubMed - indexed for MEDLINE]Related articlesFree article

Cloning and analysis of a bifunctional methyltransferase/restriction endonuclease TspGWI, the prototype of a Thermus sp. enzyme family.

Zylicz-Stachula A, Bujnicki JM, Skowron PM.

BMC Mol Biol. 2009 May 29;10:52.PMID: 19480701 [PubMed - indexed for MEDLINE]Related articlesFree article

Construction of a reading frame-independent yeast two-hybrid vector system for site-specific recombinational cloning and protein interaction screening.

Maier R, Brandner C, Hintner H, Bauer J, Onder K.

Biotechniques. 2008 Sep;45(3):235-44.PMID: 18778248 [PubMed - indexed for MEDLINE]Related articlesFree article

Construction of small-insert libraries from genomic DNA.

Hudson TJ.

Curr Protoc Hum Genet. 2001 May;Chapter 2:Unit 2.1.PMID: 18428265 [PubMed - indexed for MEDLINE]Related articles

The MmeI family: type II restriction-modification enzymes that employ single-strand modification for host protection.

Morgan RD, Dwinell EA, Bhatia TK, Lang EM, Luyten YA.

Nucleic Acids Res. 2009 Aug;37(15):5208-21. Epub 2009 Jul 3.PMID: 19578066 [PubMed - indexed for MEDLINE]Related articlesFree article

Efficient four fragment cloning for the construction of vectors for targeted gene replacement in filamentous fungi.

Frandsen RJ, Andersson JA, Kristensen MB, Giese H.

BMC Mol Biol. 2008 Aug 1;9:70.PMID: 18673530 [PubMed - indexed for MEDLINE]Related articlesFree article

Rational engineering of type II restriction endonuclease DNA binding and cleavage specificity.

Morgan RD, Luyten YA.

Nucleic Acids Res. 2009 Aug;37(15):5222-33. Epub 2009 Jun 30.PMID: 19567736 [PubMed - indexed for MEDLINE]Related articlesFree article

Domain organization and functional analysis of type IIS restriction endonuclease Eco31I.

Jakubauskas A, Sasnauskas G, Giedriene J, Janulaitis A.

Biochemistry. 2008 Aug 19;47(33):8546-56. Epub 2008 Jul 22.PMID: 18642930 [PubMed - indexed for MEDLINE]Related articles

Recursive Directional Ligation by Plasmid Reconstruction Allows Rapid and Seamless Cloning of Oligomeric Genes.

McDaniel JR, Mackay JA, Quiroz FG, Chilkoti A.

Biomacromolecules. 2010 Feb 25. [Epub ahead of print]PMID: 20184309 [PubMed - as supplied by publisher]Related articles

A versatile zero background T-vector system for gene cloning and functional genomics.

Chen S, Songkumarn P, Liu J, Wang GL.

Plant Physiol. 2009 Jul;150(3):1111-21. Epub 2009 Apr 29.PMID: 19403729 [PubMed - indexed for MEDLINE]Related articlesFree article

A universal method for directional cloning of PCR products based on asymmetric PCR.

Wang BL, Jiao YL, Li XX, Zheng F, Liang H, Sun ZY, Guo G.

Biotechnol Appl Biochem. 2009 Jan;52(Pt 1):41-4.PMID: 18352859 [PubMed - indexed for MEDLINE]Related articles