PubMed

Homologous high-throughput expression and purification of highly conserved E coli proteins.

Ergin A, Büssow K, Sieper J, Thiel A, Duchmann R, Adam T.

Microb Cell Fact. 2007 Jun 6;6:18.PMID: 17553160 [PubMed]Related articlesFree article

A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production.

Cabrita LD, Dai W, Bottomley SP.

BMC Biotechnol. 2006 Mar 1;6:12.PMID: 16509985 [PubMed - indexed for MEDLINE]Related articlesFree article

Identification of the predominant antigenic epitopes in intestinal flora in IBD.

Ergin A, Adam T, Büssow K, Thiel A, Sieper J, Duchmann R.

Mucosal Immunol. 2008 Nov;1 Suppl 1:S19-23.PMID: 19079222 [PubMed - indexed for MEDLINE]Related articles

An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography.

Scheich C, Sievert V, Büssow K.

BMC Biotechnol. 2003 Jul 28;3:12. Epub 2003 Jul 28.PMID: 12885298 [PubMed - indexed for MEDLINE]Related articlesFree article

Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors.

Dümmler A, Lawrence AM, de Marco A.

Microb Cell Fact. 2005 Dec 13;4:34.PMID: 16351710 [PubMed]Related articlesFree article

An expression vector tailored for large-scale, high-throughput purification of recombinant proteins.

Donnelly MI, Zhou M, Millard CS, Clancy S, Stols L, Eschenfeldt WH, Collart FR, Joachimiak A.

Protein Expr Purif. 2006 Jun;47(2):446-54. Epub 2006 Jan 30.PMID: 16497515 [PubMed - indexed for MEDLINE]Related articlesFree article

Expression, purification, and antibody binding activity of human papillomavirus 16 L1 protein fused to maltose binding protein.

Cho HJ, Hahm MS, Kim MK, Han IK, Jung WW, Choi HG, Kim JA, Oh YK.

Protein Pept Lett. 2007;14(5):417-24.PMID: 17584165 [PubMed - indexed for MEDLINE]Related articles

Automated production of recombinant human proteins as resource for proteome research.

Kohl T, Schmidt C, Wiemann S, Poustka A, Korf U.

Proteome Sci. 2008 Jan 28;6:4.PMID: 18226205 [PubMed]Related articlesFree article

pCold-GST vector: a novel cold-shock vector containing GST tag for soluble protein production.

Hayashi K, Kojima C.

Protein Expr Purif. 2008 Nov;62(1):120-7. Epub 2008 Jul 29.PMID: 18694833 [PubMed - indexed for MEDLINE]Related articles

Expression and purification of the complete PreS region of hepatitis B Virus.

Deng Q, Kong YY, Xie YH, Wang Y.

World J Gastroenterol. 2005 May 28;11(20):3060-4.PMID: 15918190 [PubMed - indexed for MEDLINE]Related articlesFree article

Construction of a set Gateway-based destination vectors for high-throughput cloning and expression screening in Escherichia coli.

Busso D, Delagoutte-Busso B, Moras D.

Anal Biochem. 2005 Aug 15;343(2):313-21.PMID: 15993367 [PubMed - indexed for MEDLINE]Related articles

Effect of N-terminal solubility enhancing fusion proteins on yield of purified target protein.

Hammarström M, Woestenenk EA, Hellgren N, Härd T, Berglund H.

J Struct Funct Genomics. 2006 Mar;7(1):1-14. Epub 2006 Jul 19.PMID: 16850178 [PubMed - indexed for MEDLINE]Related articles

Improving solubility of Shewanella oneidensis MR-1 and Clostridium thermocellum JW-20 proteins expressed into Esherichia coli.

Kataeva I, Chang J, Xu H, Luan CH, Zhou J, Uversky VN, Lin D, Horanyi P, Liu ZJ, Ljungdahl LG, Rose J, Luo M, Wang BC.

J Proteome Res. 2005 Nov-Dec;4(6):1942-51.PMID: 16335938 [PubMed - indexed for MEDLINE]Related articles

Maltose binding protein facilitates high-level expression and functional purification of the chemokines RANTES and SDF-1alpha from Escherichia coli.

Cho HJ, Lee Y, Chang RS, Hahm MS, Kim MK, Kim YB, Oh YK.

Protein Expr Purif. 2008 Jul;60(1):37-45. Epub 2008 Mar 30.PMID: 18455434 [PubMed - indexed for MEDLINE]Related articles

High-yield expression in Escherichia coli of soluble human MT2A with native functions.

Yang F, Zhou M, He Z, Liu X, Sun L, Sun Y, Chen Z.

Protein Expr Purif. 2007 May;53(1):186-94. Epub 2006 Dec 15.PMID: 17224279 [PubMed - indexed for MEDLINE]Related articles

Two methods for improved purification of full-length mammalian proteins that have poor expression and/or solubility using standard Escherichia coli procedures.

Geng J, Carstens RP.

Protein Expr Purif. 2006 Jul;48(1):142-50. Epub 2006 Feb 20.PMID: 16529945 [PubMed - indexed for MEDLINE]Related articles

High-throughput production of recombinant antigens for mouse KIAA proteins in Escherichia coli: computational allocation of possible antigenic regions, and construction of expression plasmids of glutathione-S-transferase-fused antigens by an in vitro recombination-assisted method.

Hara Y, Shimada K, Kohga H, Ohara O, Koga H.

DNA Res. 2003 Jun 30;10(3):129-36.PMID: 12886955 [PubMed - indexed for MEDLINE]Related articlesFree article

Cloning, expression and rapid purification of active recombinant mycothiol ligase as B1 immunoglobulin binding domain of streptococcal protein G, glutathione-S-transferase and maltose binding protein fusion proteins in Mycobacterium smegmatis.

Gutierrez-Lugo MT, Newton GL, Fahey RC, Bewley CA.

Protein Expr Purif. 2006 Nov;50(1):128-36. Epub 2006 Jul 11.PMID: 16908186 [PubMed - indexed for MEDLINE]Related articles

Large-scale protein expression for proteome research.

Korf U, Kohl T, van der Zandt H, Zahn R, Schleeger S, Ueberle B, Wandschneider S, Bechtel S, Schnölzer M, Ottleben H, Wiemann S, Poustka A.

Proteomics. 2005 Sep;5(14):3571-80.PMID: 16127724 [PubMed - indexed for MEDLINE]Related articles

The solubility and stability of recombinant proteins are increased by their fusion to NusA.

De Marco V, Stier G, Blandin S, de Marco A.

Biochem Biophys Res Commun. 2004 Sep 24;322(3):766-71.PMID: 15336530 [PubMed - indexed for MEDLINE]Related articles