PubMed

RNA mutagenesis yields highly diverse mRNA libraries for in vitro protein evolution.

Kopsidas G, Carman RK, Stutt EL, Raicevic A, Roberts AS, Siomos MA, Dobric N, Pontes-Braz L, Coia G.

BMC Biotechnol. 2007 Apr 11;7:18.PMID: 17425805 [PubMed - indexed for MEDLINE]Related articlesFree article

In vitro improvement of a shark IgNAR antibody by Qbeta replicase mutation and ribosome display mimics in vivo affinity maturation.

Kopsidas G, Roberts AS, Coia G, Streltsov VA, Nuttall SD.

Immunol Lett. 2006 Nov 15;107(2):163-8. Epub 2006 Oct 16.PMID: 17069896 [PubMed - indexed for MEDLINE]Related articles

In vitro DNA recombination by L-Shuffling during ribosome display affinity maturation of an anti-Fas antibody increases the population of improved variants.

Chodorge M, Fourage L, Ravot G, Jermutus L, Minter R.

Protein Eng Des Sel. 2008 May;21(5):343-51.PMID: 18411227 [PubMed - indexed for MEDLINE]Related articlesFree article

In vitro selection and evolution of functional proteins by using ribosome display.

Hanes J, Plückthun A.

Proc Natl Acad Sci U S A. 1997 May 13;94(10):4937-42.PMID: 9144168 [PubMed - indexed for MEDLINE]Related articlesFree article

Affinity maturation of human growth hormone by monovalent phage display.

Lowman HB, Wells JA.

J Mol Biol. 1993 Dec 5;234(3):564-78.PMID: 8254660 [PubMed - indexed for MEDLINE]Related articles

In vitro evolution of single-chain antibodies using mRNA display.

Fukuda I, Kojoh K, Tabata N, Doi N, Takashima H, Miyamoto-Sato E, Yanagawa H.

Nucleic Acids Res. 2006;34(19):e127. Epub 2006 Sep 29.PMID: 17012279 [PubMed - indexed for MEDLINE]Related articlesFree article

Increased folding stability of TEM-1 beta-lactamase by in vitro selection.

Kather I, Jakob RP, Dobbek H, Schmid FX.

J Mol Biol. 2008 Oct 31;383(1):238-51. Epub 2008 Aug 3.PMID: 18706424 [PubMed - indexed for MEDLINE]Related articles

Transversion-enriched sequence saturation mutagenesis (SeSaM-Tv+): a random mutagenesis method with consecutive nucleotide exchanges that complements the bias of error-prone PCR.

Wong TS, Roccatano D, Loakes D, Tee KL, Schenk A, Hauer B, Schwaneberg U.

Biotechnol J. 2008 Jan;3(1):74-82.PMID: 18022859 [PubMed - indexed for MEDLINE]Related articles

Combining computational and experimental screening for rapid optimization of protein properties.

Hayes RJ, Bentzien J, Ary ML, Hwang MY, Jacinto JM, Vielmetter J, Kundu A, Dahiyat BI.

Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):15926-31. Epub 2002 Nov 21.PMID: 12446841 [PubMed - indexed for MEDLINE]Related articlesFree article

Ribosome display: selecting and evolving proteins in vitro that specifically bind to a target.

Zahnd C, Amstutz P, Plückthun A.

Nat Methods. 2007 Mar;4(3):269-79.PMID: 17327848 [PubMed - indexed for MEDLINE]Related articles

Evolution of an interloop disulfide bond in high-affinity antibody mimics based on fibronectin type III domain and selected by yeast surface display: molecular convergence with single-domain camelid and shark antibodies.

Lipovsek D, Lippow SM, Hackel BJ, Gregson MW, Cheng P, Kapila A, Wittrup KD.

J Mol Biol. 2007 May 11;368(4):1024-41. Epub 2007 Feb 22.PMID: 17382960 [PubMed - indexed for MEDLINE]Related articles

In-vitro protein evolution by ribosome display and mRNA display.

Lipovsek D, Plückthun A.

J Immunol Methods. 2004 Jul;290(1-2):51-67. Review. Erratum in: J Immunol Methods. 2004 Nov;294(1-2):213. PMID: 15261571 [PubMed - indexed for MEDLINE]Related articles

Ribosome display and affinity maturation: from antibodies to single V-domains and steps towards cancer therapeutics.

Irving RA, Coia G, Roberts A, Nuttall SD, Hudson PJ.

J Immunol Methods. 2001 Feb 1;248(1-2):31-45. Review.PMID: 11223067 [PubMed - indexed for MEDLINE]Related articles

Selection for improved protein stability by phage display.

Jung S, Honegger A, Plückthun A.

J Mol Biol. 1999 Nov 19;294(1):163-80.PMID: 10556036 [PubMed - indexed for MEDLINE]Related articles

Generation of protein lineages with new sequence spaces by functional salvage screen.

Kim GJ, Cheon YH, Park MS, Park HS, Kim HS.

Protein Eng. 2001 Sep;14(9):647-54.PMID: 11707610 [PubMed - indexed for MEDLINE]Related articlesFree article

Protein design through systematic catalytic loop exchange in the (beta/alpha)8 fold.

Ochoa-Leyva A, Soberón X, Sánchez F, Argüello M, Montero-Morán G, Saab-Rincón G.

J Mol Biol. 2009 Apr 10;387(4):949-64. Epub 2009 Feb 20.PMID: 19233201 [PubMed - indexed for MEDLINE]Related articles

Site-saturation mutagenesis is more efficient than DNA shuffling for the directed evolution of beta-fucosidase from beta-galactosidase.

Parikh MR, Matsumura I.

J Mol Biol. 2005 Sep 23;352(3):621-8.PMID: 16095606 [PubMed - indexed for MEDLINE]Related articlesFree article

The effect of high-frequency random mutagenesis on in vitro protein evolution: a study on TEM-1 beta-lactamase.

Zaccolo M, Gherardi E.

J Mol Biol. 1999 Jan 15;285(2):775-83.PMID: 9878443 [PubMed - indexed for MEDLINE]Related articles

Tailoring in vitro evolution for protein affinity or stability.

Jermutus L, Honegger A, Schwesinger F, Hanes J, Plückthun A.

Proc Natl Acad Sci U S A. 2001 Jan 2;98(1):75-80.PMID: 11134506 [PubMed - indexed for MEDLINE]Related articlesFree article

Algorithms for selecting breakpoint locations to optimize diversity in protein engineering by site-directed protein recombination.

Zheng W, Ye X, Friedman AM, Bailey-Kellogg C.

Comput Syst Bioinformatics Conf. 2007;6:31-40.PMID: 17951810 [PubMed - indexed for MEDLINE]Related articlesFree article